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Literature summary for 3.5.1.2 extracted from
- Efficient expression and purification of recombinant glutaminase from Bacillus licheniformis (GlsA) in Escherichia coli (2012), Protein Expr. Purif., 83, 52-58.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | microbial glutaminases are enzymes with emerging potential in both the food and the pharmaceutical industries, potential application for bioconversion of glutamine to flavor-enhancing glutamic acid | Bacillus licheniformis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene glsA, expression of His10-tagged GlsA in Escherichia coli under the control of a ptac promoter | Bacillus licheniformis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Bacillus licheniformis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
gel filtration | Bacillus licheniformis |
| 38500 | - |
1 * 38500, about, sequence calculation, 1 * 39000, SDS-PAGE | Bacillus licheniformis |
| 39000 | - |
1 * 38500, about, sequence calculation, 1 * 39000, SDS-PAGE | Bacillus licheniformis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + H2O | Bacillus licheniformis | - |
L-glutamate + NH3 | - |
? |  |
| L-glutamine + H2O | Bacillus licheniformis DSM 13 | - |
L-glutamate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | Q65NY1 | ATCC 14580 | - |
| Bacillus licheniformis DSM 13 | Q65NY1 | ATCC 14580 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His10-tagged GlsA 2.7fold from Escherichia coli by nickel affinity chromatography to homogeneity | Bacillus licheniformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 325 | - |
purified recombinant His10-tagged GlsA, pH 7.5, 30°C | Bacillus licheniformis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 30°C, pH 7.5, purified recombinant enzyme, stable for up to 6 h | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + H2O | - |
Bacillus licheniformis | L-glutamate + NH3 | - |
? | |
| L-glutamine + H2O | - |
Bacillus licheniformis DSM 13 | L-glutamate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 38500, about, sequence calculation, 1 * 39000, SDS-PAGE | Bacillus licheniformis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlsA | - |
Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bacillus licheniformis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | glutaminase functions in cellular metabolism of every organism by supplying nitrogen required for the biosynthesis of a variety of metabolic intermediates, while glutamic acid plays a role in both sensory and nutritional properties of food | Bacillus licheniformis |
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