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Literature summary for 3.5.1.2 extracted from
- Molecular cloning, overexpression, and purification of Micrococcus luteus K-3-type glutaminase from Aspergillus oryzae RIB40 (2004), Protein Expr. Purif., 38, 272-278.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene AoglsA, from strain RIB40, DNA and amino acid sequence determination and analysis, functional overexpression in Saccharomyces cerevisiae cell wall and in Escherichia coli as soluble protein | Aspergillus oryzae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49883 | - |
x * 49883, amino acid sequence calculation | Aspergillus oryzae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + H2O | Aspergillus oryzae | - |
L-glutamate + NH3 | - |
? |  |
| L-glutamine + H2O | Aspergillus oryzae RIB 40 | - |
L-glutamate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | Q66V97 | - |
- |
| Aspergillus oryzae RIB 40 | Q66V97 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 5.9fold from Escherichia coli by anion exchange, glutamine affinity, and hydrophobic interaction chromatography to homogeneity | Aspergillus oryzae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of different enzyme constructs recombinantly expressed in different Escherichia coli strains, overview | Aspergillus oryzae |
| 11.1 | - |
recombinant enzyme in Escherichia coli strain JM109 | Aspergillus oryzae |
| 730 | - |
purified recombinant enzyme | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + H2O | - |
Aspergillus oryzae | L-glutamate + NH3 | - |
? | |
| L-glutamine + H2O | - |
Aspergillus oryzae RIB 40 | L-glutamate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49883, amino acid sequence calculation | Aspergillus oryzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AoGls | - |
Aspergillus oryzae |
| Micrococcus luteus K-3-type glutaminase | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Aspergillus oryzae |
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