Cloned (Comment) | Organism |
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GLS1 gene, cloning from genomic library, quantitative expression analysis | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of GLS1-knockout mice using W9.5 embryonic stem ES cells and C57BL6/J blastocysts, mice lacking brain/kidney phosphate-activated glutaminase have impaired glutamatergic synaptic transmission, altered breathing, disorganized goal-directed behavior and die shortly after birth, the amplitude of inspirations is decreased in vivo, chemosensitivity to CO2 is severely altered, and the frequency of pacemaker activity recorded in the respiratory generator in the in vitro isolated pre-Boetzinger complex, a glutamatergic brainstem network, is increased, phenotypic characterization of GLS1 neonate mutant mice, overview | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
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Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | Mus musculus | neurotransmitter glutamate has been thought to derive mainly from glutamine via the action of glutaminase type 1, the GLS1 pathway is essential for maintaining the function of active synapses, knockout mice lacking brain/kidney phosphate-activated glutaminase have impaired glutamatergic synaptic transmission, altered breathing, disorganized goal-directed behavior and die shortly after birth | L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | of newborn mice, and adult brain cortex and stem, GLS1 | Mus musculus | - |
central nervous system | GLS1 | Mus musculus | - |
heart | GLS1 | Mus musculus | - |
kidney | GLS1 | Mus musculus | - |
liver | neonatal liver, all GLS1 activity is caused by GLS1, which is downregulated during development, and supplanted by the liver-specific glutaminase type 2, GLS2, in adulthood | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | - |
Mus musculus | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | neurotransmitter glutamate has been thought to derive mainly from glutamine via the action of glutaminase type 1, the GLS1 pathway is essential for maintaining the function of active synapses, knockout mice lacking brain/kidney phosphate-activated glutaminase have impaired glutamatergic synaptic transmission, altered breathing, disorganized goal-directed behavior and die shortly after birth | Mus musculus | L-glutamate + NH3 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |