Literature summary for 3.5.1.2 extracted from

Kenny, J.; Bao, Y.; Hamm, B.; Taylor, L.; Toth, A.; Wagers, B.; Curthoys, N.P.
Bacterial expression, purification, and characterization of rat kidney-type mitochondrial glutaminase (2003), Protein Expr. Purif., 31, 140-148.

Search for more literature for 3.5.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.3	-	L-glutamine	recombinant enzyme with N-terminal and C-terminal deletions, T-phosphate buffer, pH 8.0	Rattus norvegicus
3.6	-	L-glutamine	recombinant enzyme with N-terminal deletion, T-phosphate buffer, pH 8.0	Rattus norvegicus
6.4	-	L-glutamine	recombinant enzyme with N-terminal and C-terminal deletions, T-acetate buffer, pH 8.0	Rattus norvegicus
7.9	-	L-glutamine	recombinant enzyme with N-terminal deletion, T-acetate buffer, pH 8.0	Rattus norvegicus
8.1	-	L-glutamine	native enzyme, T-phosphate buffer, pH 8.0	Rattus norvegicus
14	-	L-glutamine	native enzyme, T-acetate buffer, pH 8.0	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Rattus norvegicus	5739	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
phosphate	induces association to form active tetramers	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	recombinant enzyme with N-terminal and C-terminal deletions, SDS-PAGE	Rattus norvegicus
49700	-	recombinant enzyme with N-terminal and C-terminal deletions, based on amino acid content	Rattus norvegicus
63200	-	4 * 63200, SDS-PAGE, recombinant enzyme with N-terminal deletion	Rattus norvegicus
64000	-	4 * 64000, based on amino acid content, recombinant enzyme with N-terminal deletion	Rattus norvegicus
260000	-	recombinant enzyme with N-terminal deletion dialyzed versus T-phosphate buffer, pH 8.0, gel filtration	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-glutamine + H2O	Rattus norvegicus	renal ammonia genesis and the utilization of glutamine as a metabolic fuel, neuronal synthesis of glutamate	L-glutamate + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	in mitochondria, the first 16 amino acids are removed	Rattus norvegicus

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme with N-terminal deletion and recombinant enzyme with N-terminal and C-terminal deletions, by nickel-affinity chromatography, 63fold and 113fold respectively	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
403	-	recombinant enzyme with N-terminal deletion	Rattus norvegicus
405	-	recombinant enzyme with N-terminal and C-terminal deletions	Rattus norvegicus

Storage Stability

Storage Stability	Organism
4ºC, T-phosphate buffer, pH 8.0, 1 mM dithiothreitol, very stable	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamine + H2O	-	Rattus norvegicus	L-glutamate + NH3	-	?
L-glutamine + H2O	renal ammonia genesis and the utilization of glutamine as a metabolic fuel, neuronal synthesis of glutamate	Rattus norvegicus	L-glutamate + NH3	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 63200, SDS-PAGE, recombinant enzyme with N-terminal deletion	Rattus norvegicus
tetramer	4 * 64000, based on amino acid content, recombinant enzyme with N-terminal deletion	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
KGA	-	Rattus norvegicus
kidney-type-glutaminase	-	Rattus norvegicus

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Release 2023.1 (January 2023)