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Literature summary for 3.5.1.2 extracted from

  • Huerta-Saquero, A.; Calderon-Flores, A.; Diaz-Villasenor, A.; Du Pont, G.; Duran, S.
    Regulation of transcription and activity of Rhizobium etli glutaminase A (2004), Biochim. Biophys. Acta, 1673, 201-207.
    View publication on PubMed
Search for more literature for 3.5.1.2

Activating Compound

Activating Compound Comment Organism Structure
glyoxylate 50 mM, increasing enzyme activity by 30% Rhizobium etli
oxaloacetate 50 mM, increasing enzyme activity by 30% Rhizobium etli

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rhizobium etli

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate competitive inhibition Rhizobium etli
D-glucose negative influence in media with glucose as carbon source Rhizobium etli
additional information no inhibited by glutamate Rhizobium etli
pyruvate mixed inhibition Rhizobium etli

Metals/Ions

Metals/Ions Comment Organism Structure
NH4+ Ka 1 mM Rhizobium etli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + H2O Rhizobium etli enzyme participates in an ATP-consuming cycle, plays a catabolic role in the degradation of glutamine to carbon skeletons, maintaining the optimal balance between glutamine and glutamate L-glutamate + NH3
-
 ir

Organism

Organism UniProt Comment Textmining
Rhizobium etli
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification
-
 Rhizobium etli

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, by metal affinity chromatography Rhizobium etli

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Rhizobium etli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + H2O
-
 Rhizobium etli L-glutamate + NH3
-
 ?
L-glutamine + H2O enzyme participates in an ATP-consuming cycle, plays a catabolic role in the degradation of glutamine to carbon skeletons, maintaining the optimal balance between glutamine and glutamate Rhizobium etli L-glutamate + NH3
-
 ir

Synonyms

Synonyms Comment Organism
glutaminase A
-
 Rhizobium etli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.7
-
 pyruvate pH 7.0, at 37ºC Rhizobium etli
2.7
-
 2-oxoglutarate pH 7.0, at 37ºC Rhizobium etli