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Literature summary for 3.5.1.2 extracted from

  • Chantawannakul, P.; Yoshimune, K.; Shirakihara, Y.; Shiratori, A.; Wakayama, M.; Moriguchi, M.
    Crystallization and preliminary X-ray crystallographic studies of salt-tolerant glutaminase from Micrococcus luteus K-3 (2003), Acta Crystallogr. Sect. D, 59, 566-568.
    View publication on PubMed
Search for more literature for 3.5.1.2

Application

Application Comment Organism
additional information food-processing industry, increases the amount of the savory flavoring L-glutamic acid Micrococcus luteus

Cloned(Commentary)

Cloned (Comment) Organism
high-expression in Escherichia coli Micrococcus luteus

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme and its fragment containing about 80% of the protein, handing-drop vapour-diffusion method Micrococcus luteus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information equivalent activities in absence and the presence of 3 M NaCl Micrococcus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38500
-
 fragment enzyme, SDS-PAGE Micrococcus luteus
48300
-
 intact enzyme, SDS-PAGE Micrococcus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + H2O Micrococcus luteus
-
 L-glutamic acid + NH3
-
 ?
L-glutamine + H2O Micrococcus luteus K-3
-
 L-glutamic acid + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
-
-
Micrococcus luteus K-3
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification
-
 Micrococcus luteus

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl column Micrococcus luteus

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Micrococcus luteus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 1230 U/mg, original strain Micrococcus luteus
additional information
-
 1965 U/mg, recombinant protein Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + H2O
-
 Micrococcus luteus L-glutamic acid + NH3
-
 ?
L-glutamine + H2O
-
 Micrococcus luteus K-3 L-glutamic acid + NH3
-
 ?

Synonyms

Synonyms Comment Organism
glutaminase A
-
 Micrococcus luteus