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Literature summary for 3.5.1.2 extracted from
- Glutaminase from Acinetobacter glutaminsificans (1985), Methods Enzymol., 113, 257-263.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Acinetobacter glutaminasificans |
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing produces a substantial loss of activity | Acinetobacter glutaminasificans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-diazo-5-oxo-L-norleucine | - |
Acinetobacter glutaminasificans |  |
| glutamate | no inhibition | Acinetobacter glutaminasificans | |
| methylene blue | 0.005%, irreversible inactivation | Acinetobacter glutaminasificans | |
| Rose bengal | 0.01%, irreversible inactivation | Acinetobacter glutaminasificans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.8 | - |
L-Asn | - |
Acinetobacter glutaminasificans | |
| 5.8 | - |
L-Gln | - |
Acinetobacter glutaminasificans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
4 * 33000 | Acinetobacter glutaminasificans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter glutaminasificans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acinetobacter glutaminasificans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, enzyme preparation as a lyophilized powder, indefinitely stable | Acinetobacter glutaminasificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-cyanoalanine + H2O | 0.2% of the activity with L-Gln | Acinetobacter glutaminasificans | ? | - |
? | |
| D-Asn + H2O | 26% of the activity with L-Gln | Acinetobacter glutaminasificans | D-Asp + NH3 | - |
? | |
| D-Gln + H2O | 35% of the activity with L-Gln | Acinetobacter glutaminasificans | D-Glu + NH3 | - |
? | |
| Gln + H2O | - |
Acinetobacter glutaminasificans | Glu + NH3 | - |
? | |
| L-Asn + H2O | 77% of the activity with L-Gln | Acinetobacter glutaminasificans | L-Asp + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 33000 | Acinetobacter glutaminasificans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
hydrolysis of L-Asn and L-Gln | Acinetobacter glutaminasificans |
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Release 2023.1 (January 2023)
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