Literature summary for 3.5.1.18 extracted from

Reinhard, L.; Mueller-Dieckmann, J.; Weiss, M.S.
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of succinyl-diaminopimelate desuccinylase (Rv1202, DapE) from Mycobacterium tuberculosis (2012), Acta Crystallogr. Sect. F, 68, 1089-1093.

Cloned(Commentary)

Cloned (Comment)	Organism
gene dape, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) chaperone combination 3 (cc3) cells, which co-express the chaperones GroEL and GroES in order to increase the yield of soluble protein expression	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, 0.001-0.002 ml of 3-7 mg/ml protein in 50 mM Bis-Tris, pH 6.0, 200 mM NaCl, 0.5 mM TCEP is mixed with 0.001-0.002 ml reservoir solution, containing 5-10% w/v PEG 4000 or PEG 3350, 35-120 mM ammonium sulfate, 100 mM sodium acetate, pH 4.1-4.6, and equilibrated against 0.9 ml reservoir solution, at room temperature, 1 day, method optimization, X-ray diffraction structure determination and analysis at 2.4-2.58 A resolution, two crystal forms	Mycobacterium tuberculosis

Crystallization (Comment)

Organism

purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, 0.001-0.002 ml of 3-7 mg/ml protein in 50 mM Bis-Tris, pH 6.0, 200 mM NaCl, 0.5 mM TCEP is mixed with 0.001-0.002 ml reservoir solution, containing 5-10% w/v PEG 4000 or PEG 3350, 35-120 mM ammonium sulfate, 100 mM sodium acetate, pH 4.1-4.6, and equilibrated against 0.9 ml reservoir solution, at room temperature, 1 day, method optimization, X-ray diffraction structure determination and analysis at 2.4-2.58 A resolution, two crystal forms

Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-succinyl-LL-2,6-diaminoheptanedioate + H2O	Mycobacterium tuberculosis	-	succinate + LL-2,6-diaminoheptanedioate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WHS9	gene dapE or Rv1202	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-succinyl-LL-2,6-diaminoheptanedioate + H2O	-	Mycobacterium tuberculosis	succinate + LL-2,6-diaminoheptanedioate	-	?

Synonyms

Synonyms	Comment	Organism
DapE	-	Mycobacterium tuberculosis
S-DAP deacylase	-	Mycobacterium tuberculosis
succinyl-diaminopimelate desuccinylase	-	Mycobacterium tuberculosis

General Information

General Information	Comment	Organism
metabolism	the enzyme catalyzes the seventh reaction step of the succinyl pathway	Mycobacterium tuberculosis