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Literature summary for 3.4.24.7 extracted from
- Matrix metalloproteinase-1 cleavage site recognition and binding in full-length human type III collagen (2009), Matrix Biol., 28, 373-379.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 4-aminophenylmercuric acetate | MMP-1 from synovial fibroblasts is activated with 10 mM 4-aminophenylmercuric acetate in 0.1 N NaOH at 37 °C for 3 h | Homo sapiens |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | chelates the required Zn2+ | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics with wild-type and mutant FG-5015 I785P type III collagen substrates, overview | Homo sapiens | |
| 0.0013 | - |
type III collagen | pH 7.5, 25°C, wild-type substrate | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | required for activity | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | MMP-1 cleaves a specific glycine-isoleucine or glycine-leucine bond in fibrillar collagens, despite the presence of this sequence in other parts of the protein | ? | - |
? | |
| Type I collagen + H2O | Homo sapiens | - |
? | - |
? | |
| type III collagen + H2O | Homo sapiens | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | purified MMP-1 from synovial fibroblasts | Homo sapiens | - |
| fibroblast | synovial fibroblast | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | MMP-1 cleaves a specific glycine-isoleucine or glycine-leucine bond in fibrillar collagens, despite the presence of this sequence in other parts of the protein | Homo sapiens | ? | - |
? | |
| Type I collagen + H2O | - |
Homo sapiens | ? | - |
? | |
| type III collagen + H2O | - |
Homo sapiens | ? | - |
? | |
| type III collagen + H2O | mechanism of interaction and cleavage of human type III collagen by fibroblast MMP-1 using a panel of recombinant human type III collagens containing engineered sequences in the vicinity of the cleavage site around residue I785, e.g. mutant FG-5015 I785P. Structural features of cleavage site determination in collagen type III, overview | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MMP-1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.077 | - |
type III collagen | pH 7.5, 25°C, wild-type substrate | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 58 | - |
type III collagen | pH 7.5, 25°C, wild-type substrate | Homo sapiens |
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