Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | strong inhibition with 2.6% residual activity at 10 mM, enzyme activity can be regained by the addition of Co2+, Cu2+, Fe2+, Mg2+, and Zn2+ ions after chelation of ions with EDTA | Serratia marcescens | |
Fe2+ | 68.66% residual activity at 5 mM | Serratia marcescens | |
additional information | not inhibited by iodoacetic acid and phenylmethylsulfonyl fluoride | Serratia marcescens | |
Phenanthroline | strong inhibition with 4.65% residual activity at 10 mM | Serratia marcescens | |
Sn2+ | 59.07% residual activity at 5 mM | Serratia marcescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 120.1% activity at 5 mM | Serratia marcescens | |
additional information | not influenced by Ba2+, Ca2+, Cu2+, K+, Mg2+, Na+, and Ni2+ | Serratia marcescens | |
Zn2+ | 114.02% activity at 5 mM | Serratia marcescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Serratia marcescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrin + H2O | Serratia marcescens | - |
? | - |
? | |
Fibrin + H2O | Serratia marcescens RSPB11 | - |
? | - |
? | |
Fibrinogen + H2O | Serratia marcescens | the enzyme hydrolyzes the Aalpha chain in 1 min, the Bbeta chain in 5 min, and the gamma chain in more than 1 h | ? | - |
? | |
Fibrinogen + H2O | Serratia marcescens RSPB11 | the enzyme hydrolyzes the Aalpha chain in 1 min, the Bbeta chain in 5 min, and the gamma chain in more than 1 h | ? | - |
? | |
additional information | Serratia marcescens | the enzyme does not degrade urokinase, streptokinase, and tissue plasminogen activator | ? | - |
? | |
additional information | Serratia marcescens RSPB11 | the enzyme does not degrade urokinase, streptokinase, and tissue plasminogen activator | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | - |
- |
- |
Serratia marcescens RSPB11 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G-75 gel filtration | Serratia marcescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the crude enzyme shows specific activity of 814.7 units/mg, the 15.2fold purified enzyme shows specific activity of 12365.9 units/mg. One unit of proteolytic enzyme activity is defined as the amount of enzyme that liberates 0.001 mg tyrosine per ml per minute from casein, at pH 7.5 and 37°C | Serratia marcescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | Km of 1.261 mg/ml for casein | Serratia marcescens | ? | - |
? | |
casein + H2O | Km of 1.261 mg/ml for casein | Serratia marcescens RSPB11 | ? | - |
? | |
Fibrin + H2O | - |
Serratia marcescens | ? | - |
? | |
Fibrin + H2O | - |
Serratia marcescens RSPB11 | ? | - |
? | |
Fibrinogen + H2O | the enzyme hydrolyzes the Aalpha chain in 1 min, the Bbeta chain in 5 min, and the gamma chain in more than 1 h | Serratia marcescens | ? | - |
? | |
Fibrinogen + H2O | the enzyme hydrolyzes the Aalpha chain in 1 min, the Bbeta chain in 5 min, and the gamma chain in more than 1 h | Serratia marcescens RSPB11 | ? | - |
? | |
additional information | the enzyme does not degrade urokinase, streptokinase, and tissue plasminogen activator | Serratia marcescens | ? | - |
? | |
additional information | the enzyme does not degrade urokinase, streptokinase, and tissue plasminogen activator | Serratia marcescens RSPB11 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Serratia marcescens |
Synonyms | Comment | Organism |
---|---|---|
Serratia marcescens metalloproteinase | - |
Serratia marcescens |
SlpB | - |
Serratia marcescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Serratia marcescens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | more than 50% activity between 30 and 50°C | Serratia marcescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 70 | after 1 h of incubation, the enzyme shows 100% activity between 4 and 37°C, 95% activity at 40°C, 85% activity at 50°C, 65% activity at 60°C, and 50% activity at 70°C | Serratia marcescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Serratia marcescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | more than 50% activity between pH 7.0 and 11.0 | Serratia marcescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | the enzyme shows more than 50% activity at pH 6.0-10.0 after 1 h incubation time, while residual activities ranging from 43.20-8.30% are observed at higher pH (11.0-12.0) and lower pH (4.0-5.0) values | Serratia marcescens |