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Literature summary for 3.4.24.35 extracted from

  • Geurts, N.; Martens, E.; Van Aelst, I.; Proost, P.; Opdenakker, G.; Van den Steen, P.E.
    Beta-hematin interaction with the hemopexin domain of gelatinase B/MMP-9 provokes autocatalytic processing of the propeptide, thereby priming activation by MMP-3 (2008), Biochemistry, 47, 2689-2699.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information beta-hematin, and not hemin, accelerates the activation of MMP-9, activation of MMP-9 by hemin and MMP-3, overview Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of full-length proMMP-9 and of mutant enzymes in Spodoptera frugiperda Sf9 insect cells using the baculovirus infection system Homo sapiens

Protein Variants

Protein Variants Comment Organism
E402A site-directed mutagenesis of the catalytic Glu402 results in an inactive enzyme Homo sapiens
additional information construction of proteolytically inactive truncated MMP-9 mutant: MMP-9DELTAHem, lacking the hemopexin domain, MMP-9DELTAOG, lacking the OG domain, and MMP-9DELTAOGHem, lacking both the hemopexin and the OG domain Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Homo sapiens
o-phenanthroline
-
Homo sapiens
TIMP-1
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Homo sapiens
Zn2+
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
x * 92000, full-length pro-MMP-9, SDS-PAGE, x * 82000, processed active MMP-9, SDS-PAGE Homo sapiens
92000
-
x * 92000, full-length pro-MMP-9, SDS-PAGE, x * 82000, processed active MMP-9, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Gelatin + H2O Homo sapiens
-
?
-
?
additional information Homo sapiens MMP-9 is activated by monomeric hemin and by MMP-3 cleaving at Glu40-Met41 and Arg87-Phe88, and at Glu40-Met41 and Leu52-Leu53, respectively, no effect by hemoglobin, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification MMP-3 and hemin activate MMP-9 cleaving at Glu40-Met41 and Arg87-Phe88, and at Glu40-Met41 and Leu52-Leu53, respectively, overview Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant full-length proMMP-9 and mutant enzymes from Spodoptera frugiperda Sf9 insect cells Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gelatin + H2O
-
Homo sapiens ?
-
?
Gelatin + H2O gelatin zymography Homo sapiens ?
-
?
additional information MMP-9 is activated by monomeric hemin and by MMP-3 cleaving at Glu40-Met41 and Arg87-Phe88, and at Glu40-Met41 and Leu52-Leu53, respectively, no effect by hemoglobin, overview Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 92000, full-length pro-MMP-9, SDS-PAGE, x * 82000, processed active MMP-9, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
MMP-9
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens