Literature summary for 3.4.24.27 extracted from

Ke, Q.; Chen, A.; Minoda, M.; Yoshida, H.
Safety evaluation of a thermolysin enzyme produced from Geobacillus stearothermophilus (2013), Food Chem. Toxicol., 59, 541-548.

Search for more literature for 3.4.24.27

Application

Application	Comment	Organism
additional information	thermolysin powder is not acutely toxic with an oral LD50 of more than 18,000 mg/kg (2520 mg/kg thermolysin protein) in rats and more than 24,000 mg/kg (3360 mg/kg protein) in mice. Subchronic feeding studies in rats for 91 days at doses up to 1000 mg/kg (390 mg/kg protein) revealed no significant differences between treated and non-treated groups, no evidence from allergenicity sequence analysis that thermolysin is an allergen, thermolysin is safe for use in food production	Geobacillus stearothermophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	a zinc metalloprotease	Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34400	-	x * 34400	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	formerly Bacillus stearothermophilus	-

Subunits

Subunits	Comment	Organism
?	x * 34400	Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	thermostable neutral protease	Geobacillus stearothermophilus

General Information

General Information	Comment	Organism
additional information	a thermostable neutral protease	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)