Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-chloroacetyl-N-hydroxyleucine methyl ester | irreversible inhibition | Bacillus cereus | |
N-chloroacetyl-N-hydroxyleucine methyl ester | irreversible inhibition | Bacillus thermoproteolyticus | |
N-chloroacetyl-N-hydroxyleucyl-alanyl-glycinamide | irreversible inhibition | Bacillus cereus | |
N-chloroacetyl-N-hydroxyleucyl-alanyl-glycinamide | irreversible inhibition | Bacillus thermoproteolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | thermolysin has four calcium ions responsible for its thermostability | Bacillus cereus | |
Ca2+ | thermolysin has four calcium ions responsible for its thermostability | Bacillus thermoproteolyticus | |
Zn2+ | contains Zn2+ | Bacillus cereus | |
Zn2+ | contains Zn2+ | Bacillus thermoproteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus thermoproteolyticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the major site for thermolysin cleavage specificity, (the S1' site), accepts large hydrophobic residues. Thermolysin preferentially cleaves at the N-terminal side of hydrophobic or bulky amino side chains such as Leu, Phe, Ile and Val. Thermolysin also cleaves bonds of Met, His, Tyr, Ala, Asn, Ser, Thr, Gly, Lys, Glu or Asp at the P1' site | Bacillus cereus | ? | - |
? | |
additional information | the major site for thermolysin cleavage specificity, (the S1' site), accepts large hydrophobic residues. Thermolysin preferentially cleaves at the N-terminal side of hydrophobic or bulky amino side chains such as Leu, Phe, Ile and Val. Thermolysin also cleaves bonds of Met, His, Tyr, Ala, Asn, Ser, Thr, Gly, Lys, Glu or Asp at the P1' site | Bacillus thermoproteolyticus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
82 | - |
the enzyme loses 50% of its activity after 30 min at 82°C | Bacillus thermoproteolyticus |