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Literature summary for 3.4.24.27 extracted from

  • Marguerre, A.K.; Kraemer, R.
    Lanthanide-based fluorogenic peptide substrate for the highly sensitive detection of thermolysin (2009), Bioorg. Med. Chem. Lett., 19, 5757-5759.
    View publication on PubMed
Search for more literature for 3.4.24.27

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 (7-methoxycoumarin-4yl) acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diamino-propionyl]-L-Ala-L-Arg-NH2 pH and temperature not specified in the publication Bacillus thermoproteolyticus
0.00449
-
 (europium(III) complex of a modified terpyridine)-K1K2K2GFSAK2K-black hole quencher 2 pH and temperature not specified in the publication Bacillus thermoproteolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Bacillus thermoproteolyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-carbobenzoxy-L-Asp-L-Phe-methyl ester + H2O Bacillus thermoproteolyticus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus thermoproteolyticus
-
 subspecies Bacillus thermoproteolyticus rokko
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4yl) acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diamino-propionyl]-L-Ala-L-Arg-NH2 + H2O
-
 Bacillus thermoproteolyticus ?
-
 ?
(europium(III) complex of a modified terpyridine)-K1K1K1-GFSAK1K1K-black hole quencher 2 + H2O thermolysin cleaves the substrates at the glycine-phenylalanine bond Bacillus thermoproteolyticus ?
-
 ?
(europium(III) complex of a modified terpyridine)-K1K2K2GFSAK2K-black hole quencher 2 + H2O thermolysin cleaves the substrates at the glycine-phenylalanine bond Bacillus thermoproteolyticus ?
-
 ?
(europium(III) complex of a modified terpyridine)-K1K2K2GFSAK2K2K-black hole quencher 2 + H2O thermolysin cleaves the substrates at the glycine-phenylalanine bond Bacillus thermoproteolyticus ?
-
 ?
N-carbobenzoxy-L-Asp-L-Phe-methyl ester + H2O
-
 Bacillus thermoproteolyticus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.36
-
 (7-methoxycoumarin-4yl) acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diamino-propionyl]-L-Ala-L-Arg-NH2 pH and temperature not specified in the publication Bacillus thermoproteolyticus
2.6
-
 (europium(III) complex of a modified terpyridine)-K1K2K2GFSAK2K-black hole quencher 2 pH and temperature not specified in the publication Bacillus thermoproteolyticus