Any feedback?
Literature summary for 3.4.24.27 extracted from
- Use of thermolysin in the diagnosis of prion diseases (2007), Mol. Biotechnol., 35, 161-170.
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | thermolysin is used in the diagnosis of prion diseases ovine scrapie and bovine spongiform encephalopathy, with similar sensitivity compared to proteinase K digestion, use of a protease to distinguish PrPC from PrPSc, overview | Bacillus thermoproteolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus thermoproteolyticus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | digestion of brain homogenates from healthy and scrapie-affected sheep as well as healthy and BSE-affected cattle, the PK digestion results in the amino-terminal truncation of PrPSc-producing PrP species with molecular weights of approx 17, 21, and 27 kDa that can only be detected with antibodies binding to the carboxy-terminal region of PrP, the so-called protease-resistant core or PrP27-30, overview | Bacillus thermoproteolyticus | ? | - |
? |  |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
assay at | Bacillus thermoproteolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Bacillus thermoproteolyticus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
