Cloned (Comment) | Organism |
---|---|
gene npr, DNA and amino acid sequence determination, expression of wild-type and mutant enzymes in Escherichia coli K12 strain JM109 | Bacillus thermoproteolyticus |
Protein Variants | Comment | Organism |
---|---|---|
G8C/N60C/S65P | site-directed mutagenesis, the mutant shows a similar catalytic efficiency compared tot he wild-type enzyme | Bacillus thermoproteolyticus |
G8C/N60C/S65P/L144S | site-directed mutagenesis, the mutant shows about 6fold increased catalytic effiency compared to the wild-type enzyme | Bacillus thermoproteolyticus |
L144S | site-directed mutagenesis, the mutant shows about 10fold increased catalytic effiency compared to the wild-type enzyme | Bacillus thermoproteolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and thermodynamics of wild-type and mutant enzymes in absence or presence of salt, overview | Bacillus thermoproteolyticus | |
0.06 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | pH 7.0, 25°C, recombinant mutant L144S | Bacillus thermoproteolyticus | |
0.1 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | pH 7.0, 25°C, recombinant mutant G8C/N60C/S65P/L144S | Bacillus thermoproteolyticus | |
0.18 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | pH 7.0, 25°C, recombinant mutant G8C/N60C/S65P | Bacillus thermoproteolyticus | |
0.33 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | pH 7.0, 25°C, recombinant wild-type enzyme | Bacillus thermoproteolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | four ions per enzyme molecule required for stability | Bacillus thermoproteolyticus | |
NaCl | activates | Bacillus thermoproteolyticus | |
Zn2+ | one ion per enzyme molecule required for activity | Bacillus thermoproteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thermoproteolyticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
N-carbobenzoxy-L-Asp-L-Phe methyl ester + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide + H2O | i.e. FAGLA | Bacillus thermoproteolyticus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bacillus thermoproteolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | pH 7.0, 25°C, recombinant mutant G8C/N60C/S65P | Bacillus thermoproteolyticus | |
3.4 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | pH 7.0, 25°C, recombinant wild-type enzyme | Bacillus thermoproteolyticus | |
6.7 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | pH 7.0, 25°C, recombinant mutant L144S | Bacillus thermoproteolyticus | |
7 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | pH 7.0, 25°C, recombinant mutant G8C/N60C/S65P/L144S | Bacillus thermoproteolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Bacillus thermoproteolyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile | Bacillus thermoproteolyticus |