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Literature summary for 3.4.24.27 extracted from
- Kinetics of the action of thermolysin on peptide substrates (1978), Biochemistry, 17, 3562-3568.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Bacillus thermoproteolyticus | |
| 0.55 | 1.52 | Benzyloxycarbonyl-Phe-Leu-Ala | depending on mode of preparation of the form of enzyme | Bacillus thermoproteolyticus |  |
| 0.65 | 0.72 | Benzyloxycarbonyl-(4-nitro)Phe-Leu-Ala | depending on mode of preparation of the form of enzyme | Bacillus thermoproteolyticus | |
| 0.98 | 1.39 | Benzyloxycarbonyl-Gly-Phe-Leu-Ala | depending on mode of preparation of the form of enzyme | Bacillus thermoproteolyticus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus thermoproteolyticus | - |
commercial available thermolysin | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-(4-nitro)Phe-Leu-Ala + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
| benzyloxycarbonyl-Gly-(4-nitro)Phe-Leu-Ala + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
| benzyloxycarbonyl-Gly-Gly-(4-nitro)Phe-Leu-Ala + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
| benzyloxycarbonyl-Gly-Gly-Phe-Leu-Ala + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
| benzyloxycarbonyl-Gly-Phe-Leu-Ala + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
| benzyloxycarbonyl-Phe-Leu-Ala + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 362 | 605 | Benzyloxycarbonyl-Phe-Leu-Ala | depending on mode of preparation of the form of enzyme | Bacillus thermoproteolyticus | |
| 407 | 968 | Benzyloxycarbonyl-Gly-Phe-Leu-Ala | depending on mode of preparation of the form of enzyme | Bacillus thermoproteolyticus | |
| 861 | 951 | Benzyloxycarbonyl-(4-nitro)Phe-Leu-Ala | depending on mode of preparation of the form of enzyme | Bacillus thermoproteolyticus | |
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