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Literature summary for 3.4.24.23 extracted from

  • Muta, Y.; Inouye, K.
    Tyr219 of human matrix metalloproteinase 7 is not critical for catalytic activity, but is involved in the broad pH-dependence of the activity (2011), J. Biochem., 150, 183-188.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
Y193F site-directed mutagenesis, the mutant shows no pH-dependence shift, as does mutants Y219X Homo sapiens
Y216F site-directed mutagenesis, the mutant shows no pH-dependence shift, as does mutants Y219X Homo sapiens
Y219A site-directed mutagenesis, the mutant variant retains its catalytic activity, but exhibits narrower pH-dependence than the wild-type MMP-7 Homo sapiens
Y219C site-directed mutagenesis, the mutant variant retains its catalytic activity, but exhibits narrower pH-dependence than the wild-type MMP-7 Homo sapiens
Y219D site-directed mutagenesis, the mutant variant retains its catalytic activity, but exhibits narrower pH-dependence than the wild-type MMP-7 Homo sapiens
Y219F site-directed mutagenesis, the mutant variant retains its catalytic activity, but exhibits narrower pH-dependence than the wild-type MMP-7 Homo sapiens
Y219S site-directed mutagenesis, the mutant variant retains its catalytic activity, but exhibits narrower pH-dependence than the wild-type MMP-7 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0511
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219C Homo sapiens
0.0732
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219F Homo sapiens
0.0736
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, wild-type enzyme Homo sapiens
0.0788
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219A Homo sapiens
0.0865
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219D Homo sapiens
0.211
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219S Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl wild-type and Y219F mutant MMP-7 activity is enhanced with increasing concentration of NaCl, activation by 550% and 850% at 4 M NaCl, respectively Homo sapiens
Zn2+ MMP-7 is a zinc metalloprotease. The stabilizing protein/zinc-bound water molecules might be the ionizable group responsible for pKe2 inb MMP-7 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 + H2O
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
Matrix metalloproteinase 7
-
Homo sapiens
MMP-7
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.61
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219C Homo sapiens
3.53
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219A Homo sapiens
5.41
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219S Homo sapiens
6.18
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219F Homo sapiens
6.35
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219D Homo sapiens
6.43
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
the pKe1 and pKe2 values of Y193F are 4.4 and 9.8, respectively, and those of Y216F are 4.3 and 9.8, respectively, both of which are the same as those of the wild-type enzyme with 4.2 and 9.7, respectively Homo sapiens
7.5
-
assay at Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 9 maximal activity range of wild-type enzyme, and mutants Y193F and Y216F Homo sapiens

General Information

General Information Comment Organism
evolution the active-site tyrosyl residue, Tyr219, is conserved in all other MMPs Homo sapiens
additional information one active-site tyrosyl residue is Tyr219, but Tyr219 is not the ionizable group responsible for pKe2. Tyr219 is not critical for catalytic activity, but is involved in the broad pH-dependence of the activity. Tyr193 and Tyr216 are neither critical for catalytic activity nor involved in the broad pH-dependence of the activity. There are no lysine and arginine residues in the active site of MMP-7 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
26
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219S Homo sapiens
32
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219C Homo sapiens
45
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219A Homo sapiens
74
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219D Homo sapiens
84
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219F Homo sapiens
87
-
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, wild-type enzyme Homo sapiens