Protein Variants | Comment | Organism |
---|---|---|
Y193F | site-directed mutagenesis, the mutant shows no pH-dependence shift, as does mutants Y219X | Homo sapiens |
Y216F | site-directed mutagenesis, the mutant shows no pH-dependence shift, as does mutants Y219X | Homo sapiens |
Y219A | site-directed mutagenesis, the mutant variant retains its catalytic activity, but exhibits narrower pH-dependence than the wild-type MMP-7 | Homo sapiens |
Y219C | site-directed mutagenesis, the mutant variant retains its catalytic activity, but exhibits narrower pH-dependence than the wild-type MMP-7 | Homo sapiens |
Y219D | site-directed mutagenesis, the mutant variant retains its catalytic activity, but exhibits narrower pH-dependence than the wild-type MMP-7 | Homo sapiens |
Y219F | site-directed mutagenesis, the mutant variant retains its catalytic activity, but exhibits narrower pH-dependence than the wild-type MMP-7 | Homo sapiens |
Y219S | site-directed mutagenesis, the mutant variant retains its catalytic activity, but exhibits narrower pH-dependence than the wild-type MMP-7 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0511 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, mutant Y219C | Homo sapiens | |
0.0732 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, mutant Y219F | Homo sapiens | |
0.0736 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, wild-type enzyme | Homo sapiens | |
0.0788 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, mutant Y219A | Homo sapiens | |
0.0865 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, mutant Y219D | Homo sapiens | |
0.211 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, mutant Y219S | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | wild-type and Y219F mutant MMP-7 activity is enhanced with increasing concentration of NaCl, activation by 550% and 850% at 4 M NaCl, respectively | Homo sapiens | |
Zn2+ | MMP-7 is a zinc metalloprotease. The stabilizing protein/zinc-bound water molecules might be the ionizable group responsible for pKe2 inb MMP-7 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Matrix metalloproteinase 7 | - |
Homo sapiens |
MMP-7 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.61 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, mutant Y219C | Homo sapiens | |
3.53 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, mutant Y219A | Homo sapiens | |
5.41 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, mutant Y219S | Homo sapiens | |
6.18 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, mutant Y219F | Homo sapiens | |
6.35 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, mutant Y219D | Homo sapiens | |
6.43 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the pKe1 and pKe2 values of Y193F are 4.4 and 9.8, respectively, and those of Y216F are 4.3 and 9.8, respectively, both of which are the same as those of the wild-type enzyme with 4.2 and 9.7, respectively | Homo sapiens |
7.5 | - |
assay at | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | maximal activity range of wild-type enzyme, and mutants Y193F and Y216F | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the active-site tyrosyl residue, Tyr219, is conserved in all other MMPs | Homo sapiens |
additional information | one active-site tyrosyl residue is Tyr219, but Tyr219 is not the ionizable group responsible for pKe2. Tyr219 is not critical for catalytic activity, but is involved in the broad pH-dependence of the activity. Tyr193 and Tyr216 are neither critical for catalytic activity nor involved in the broad pH-dependence of the activity. There are no lysine and arginine residues in the active site of MMP-7 | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, mutant Y219S | Homo sapiens | |
32 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, mutant Y219C | Homo sapiens | |
45 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, mutant Y219A | Homo sapiens | |
74 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, mutant Y219D | Homo sapiens | |
84 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, mutant Y219F | Homo sapiens | |
87 | - |
(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 | pH 7.5, 25°C, wild-type enzyme | Homo sapiens |