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Literature summary for 3.4.24.23 extracted from

  • Crabbe, T.; Willenbrock, F.; Eaton, D.; Hynds, P.; Carne, A.F.; Murphy, G.; Docherty, A.J.P.
    Biochemical characterization of matrilysin. Activation conforms to the stepwise mechanisms proposed for other matrix metalloproteinases (1992), Biochemistry, 31, 8500-8507.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Homo sapiens
Tissue inhibitor of metalloproteinase-1
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc contains 1 zinc atom per molecule which is essential for catalytic acivity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
x * 19000, human, active form, SDS-PAGE under reducing condition Homo sapiens
28000
-
x * 28000, human, proenzyme, SDS-PAGE under reducing condition Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
expression in Escherichia coli
-
Homo sapiens
-
expression by transfected mouse myeloma cells
-

Purification (Commentary)

Purification (Comment) Organism
latent precursor purified from transfected mouse myeloma cell conditioned medium, activation by 4-aminophenylmercuric acetate, trypsin and incubation at elevated temperatures Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
culture medium transfected mouse myeloma cell conditioned Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-Dinitrophenyl-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH2 + H2O
-
Homo sapiens 2,4-Dinitrophenyl-Pro-Leu-Gly + Leu-Trp-Ala-D-Arg-NH2
-
?

Subunits

Subunits Comment Organism
? x * 19000, human, active form, SDS-PAGE under reducing condition Homo sapiens
? x * 28000, human, proenzyme, SDS-PAGE under reducing condition Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
2,4-dinitrophenyl-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8.5 4.5: about 40% of activity maximum, 8.5: about 65% of activity maximum, 2,4-dinitrophenyl-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg Homo sapiens