Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Homo sapiens | |
Tissue inhibitor of metalloproteinase-1 | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | contains 1 zinc atom per molecule which is essential for catalytic acivity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
x * 19000, human, active form, SDS-PAGE under reducing condition | Homo sapiens |
28000 | - |
x * 28000, human, proenzyme, SDS-PAGE under reducing condition | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
expression in Escherichia coli | - |
Homo sapiens | - |
expression by transfected mouse myeloma cells | - |
Purification (Comment) | Organism |
---|---|
latent precursor purified from transfected mouse myeloma cell conditioned medium, activation by 4-aminophenylmercuric acetate, trypsin and incubation at elevated temperatures | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | transfected mouse myeloma cell conditioned | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-Dinitrophenyl-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH2 + H2O | - |
Homo sapiens | 2,4-Dinitrophenyl-Pro-Leu-Gly + Leu-Trp-Ala-D-Arg-NH2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 19000, human, active form, SDS-PAGE under reducing condition | Homo sapiens |
? | x * 28000, human, proenzyme, SDS-PAGE under reducing condition | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
2,4-dinitrophenyl-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | 4.5: about 40% of activity maximum, 8.5: about 65% of activity maximum, 2,4-dinitrophenyl-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg | Homo sapiens |