Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Astacus astacus |
Crystallization (Comment) | Organism |
---|---|
proastacin is crystallized by the sitting drop vapor diffusion method, using 20% (w/v) polyethylene glycol 8000, 0.1 M (NH4)2SO4, 0.01 M MgCl2, 0.05 M MES, pH 5.6 | Astacus astacus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required for activity | Astacus astacus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Collagen + H2O | Astacus astacus | - |
? | - |
? | |
Gelatin + H2O | Astacus astacus | - |
? | - |
? | |
additional information | Astacus astacus | optimal astacin substrates comprise at least five amino acids with small aliphatic residues in P'1, proline in P'2, bulky hydrophobic residues in P'3, and basic residues in P1 and P2 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Astacus astacus | Q9U918 | European crayfish | - |
Purification (Comment) | Organism |
---|---|
proastacin is purified by Ni-NTA column chromatography and HEMA-Bio gel filtration | Astacus astacus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
midgut gland | astacin is synthesized as a preproenzyme in the midgut gland | Astacus astacus | - |
pancreatic duct | the proenzyme is only transiently found within the ducts between the hepatopancreas and the stomach | Astacus astacus | - |
stomach | mature enzyme | Astacus astacus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Collagen + H2O | - |
Astacus astacus | ? | - |
? | |
Gelatin + H2O | - |
Astacus astacus | ? | - |
? | |
additional information | optimal astacin substrates comprise at least five amino acids with small aliphatic residues in P'1, proline in P'2, bulky hydrophobic residues in P'3, and basic residues in P1 and P2 | Astacus astacus | ? | - |
? |