Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acetyl-Arg-Pro-Gly-Tyr-hydroxamate | - |
Astacus astacus | |
actinonin | i.e. 3-[[1-[[2-(hydroxymethyl)-1-pyrolidinyl]carbonyl]-2-methylpropyl]carbamoyl]octano hydroxamic acid, weak, kinetics | Astacus astacus | |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg | nitrobradykinin as substrate | Astacus astacus | |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Glu-Pro-Phe-Arg | nitrobradykinin as substrate | Astacus astacus | |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg | nitrobradykinin as substrate | Astacus astacus | |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg | nitrobradykinin as substrate | Astacus astacus | |
Hydroxamylsuccinyl-Pro-Phe-Arg | - |
Astacus astacus | |
additional information | most effective inhibitors are hydroxamates with aromatic side chains followed in order of decreasing affinity by those with side chains containing sulfur, acidic or basic groups | Astacus astacus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
2-Aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg | - |
Astacus astacus | |
0.044 | - |
2-Aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg | - |
Astacus astacus | |
0.085 | - |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg | - |
Astacus astacus | |
0.116 | - |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg | - |
Astacus astacus | |
0.154 | - |
2-Aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg | - |
Astacus astacus | |
0.194 | - |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg | - |
Astacus astacus | |
0.209 | - |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg | - |
Astacus astacus | |
0.281 | - |
2-Aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg | - |
Astacus astacus | |
0.306 | - |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg | - |
Astacus astacus | |
0.327 | - |
2-Aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg | - |
Astacus astacus | |
0.867 | 0.87 | 2-Aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg | - |
Astacus astacus | |
0.954 | - |
2-Aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-(4-nitro)Phe-Arg | - |
Astacus astacus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | requirement | Astacus astacus | |
Zn2+ | zinc metalloenzyme | Astacus astacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Astacus astacus | - |
i.e. Astacus fluviatilis | - |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O | fluorogenic bradykinin analog substrate, cleavage site: Phe-Ser | Astacus astacus | 2-aminobenzoyl-Arg-Gly-Pro-Phe + Ser-Pro-(4-nitro)Phe-Arg | - |
? | |
2-aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O | fluorogenic bradykinin analog substrate, cleavage site: Phe-Ser | Astacus astacus | 2-aminobenzoyl-Arg-Hyp-Gly-Phe + Ser-Pro-(4-nitro)Phe-Arg | - |
? | |
2-aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg + H2O | fluorogenic bradykinin analog substrate, cleavage sites: Gly-Ala and Ala-Ser | Astacus astacus | 2-aminobenzoyl-Arg-Pro-Gly-Ala + Ser-Pro-(4-nitro)Phe-Arg | major products | ? | |
2-aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-(4-nitro)Phe-Arg + H2O | fluorogenic bradykinin analog substrate, cleavage site: Glu-Ser | Astacus astacus | 2-aminobenzoyl-Arg-Pro-Gly-Glu + Ser-Pro-(4-nitro)Phe-Arg | - |
? | |
2-aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg + H2O | fluorogenic bradykinin analog substrate, cleavage site: Leu-Ser | Astacus astacus | 2-aminobenzoyl-Arg-Pro-Gly-Leu + Ser-Pro-(4-nitro)Phe-Arg | - |
? | |
2-aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg + H2O | fluorogenic bradykinin analog substrate, cleavage site: Lys-Ser | Astacus astacus | 2-aminobenzoyl-Arg-Pro-Gly-Lys + Ser-Pro-(4-nitro)Phe-Arg | - |
? | |
2-aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O | best of fluorogenic bradykinin analog substrates, cleavage site: Phe-Ser | Astacus astacus | 2-aminobenzoyl-Arg-Pro-Ile-Phe + Ser-Pro-(4-nitro)Phe-Arg | - |
? | |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg + H2O | chromogenic bradykinin analog | Astacus astacus | Arg-Pro-Pro-Gly-(4-nitro)Phe + Ala-Pro-Phe-Arg | - |
? | |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg + H2O | chromogenic bradykinin analog | Astacus astacus | Arg-Pro-Pro-Gly-(4-nitro)Phe + Arg-Pro-Phe-Arg | - |
? | |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg + H2O | chromogenic bradykinin analog | Astacus astacus | Arg-Pro-Pro-Gly-(4-nitro)Phe + Lys-Pro-Phe-Arg | - |
? | |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg + H2O | chromogenic bradykinin analog | Astacus astacus | Arg-Pro-Pro-Gly-(4-nitro)Phe + Phe-Pro-Phe-Arg | - |
? | |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg + H2O | i.e. nitrobradykinin, chromogenic bradykinin analog | Astacus astacus | Arg-Pro-Pro-Gly-(4-nitro)Phe + Ser-Pro-Phe-Arg | - |
? | |
azocasein + H2O | - |
Astacus astacus | ? | - |
? | |
bradykinin + H2O | i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | Astacus astacus | Arg-Pro-Pro-Gly-Phe + Ser-Pro-Phe-Arg | - |
? | |
additional information | No hydrolysis of Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg, Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg, Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg, Arg-Pro-Pro-Gly-(4-nitro)Phe-Glu-Pro-Phe-Arg | Astacus astacus | ? | - |
? | |
Succinyl-Ala-Ala-Ala 4-nitroanilide + H2O | - |
Astacus astacus | Succinyl-Ala-Ala-Ala + p-nitroaniline | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Astacus astacus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg | - |
Astacus astacus | |
0.002 | - |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg | - |
Astacus astacus | |
0.004 | - |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg | - |
Astacus astacus | |
2.8 | - |
2-aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-4-nitrophenylalanine-Arg | - |
Astacus astacus | |
3.6 | - |
2-Aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg | - |
Astacus astacus | |
7.1 | - |
2-Aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg | - |
Astacus astacus | |
14.3 | - |
2-Aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg | - |
Astacus astacus | |
15.1 | - |
2-Aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg | - |
Astacus astacus | |
44.4 | - |
2-Aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg | - |
Astacus astacus | |
78.4 | - |
2-Aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg | - |
Astacus astacus | |
78.7 | - |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg | - |
Astacus astacus | |
834 | - |
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg | - |
Astacus astacus |