Literature summary for 3.4.24.15 extracted from

Nakano, H.; Hosokawa, A.; Tagawa, R.; Inaka, K.; Ohta, K.; Nakatsu, T.; Kato, H.; Watanabe, K.
Crystallization and preliminary X-ray crystallographic analysis of Pz peptidase B from Geobacillus collagenovorans MO-1 (2012), Acta Crystallogr. Sect. F, 68, 757-759.

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Cloned(Commentary)

Cloned (Comment)	Organism
recombinant enzyme expression in Escherichia coli strain BL21 (DE3)	Geobacillus sp.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant wild-type and selenomethionine-substituted enzymes, hanging-drop and sitting-drop vapour-diffusion methods, mixing of 0.002 ml of 20 mg/ml protein in 50 mM Tris-HCl, pH 7.5, in a 1:1 ratio with each of the crystallization solutions, optimal in 0.1 M bis-Tris-HCl pH 5.9, 2.3 M ammonium sulfate at 24°C, within 5 days, method optimization, ammonium sulfate after 3 days, X-ray diffraction structure determination and analysis at 1.6 A resolution	Geobacillus sp.

Crystallization (Comment)

Organism

purified recombinant wild-type and selenomethionine-substituted enzymes, hanging-drop and sitting-drop vapour-diffusion methods, mixing of 0.002 ml of 20 mg/ml protein in 50 mM Tris-HCl, pH 7.5, in a 1:1 ratio with each of the crystallization solutions, optimal in 0.1 M bis-Tris-HCl pH 5.9, 2.3 M ammonium sulfate at 24°C, within 5 days, method optimization, ammonium sulfate after 3 days, X-ray diffraction structure determination and analysis at 1.6 A resolution

Geobacillus sp.

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Geobacillus sp.	Q4W802	gene pz-B	-
Geobacillus sp.	Q4W803	gene pz-A	-
Geobacillus sp. MO-1	Q4W802	gene pz-B	-
Geobacillus sp. MO-1	Q4W803	gene pz-A	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain BL21 (DE3) by anion exchange chromatography and gel filtration	Geobacillus sp.

Synonyms

Synonyms	Comment	Organism
Pz peptidase A	-	Geobacillus sp.
Pz peptidase B	-	Geobacillus sp.

General Information

General Information	Comment	Organism
evolution	Pz peptidase A is an intracellular M3 metallopeptidase that is found together with Pz peptidase B in the thermophile Geobacillus collagenovorans MO-1 and recognizes collagen-specific tripeptide units (-Gly-Pro-X-). The peptidases have low homology in their primary structures, but their cleavage patterns towards peptide substrates are similar	Geobacillus sp.
evolution	Pz peptidase B is an intracellular M3 metallopeptidase that is found together with Pz peptidase A in the thermophile Geobacillus collagenovorans MO-1 and recognizes collagen-specific tripeptide units (-Gly-Pro-X-). The peptidases have low homology in their primary structures, but their cleavage patterns towards peptide substrates are similar. The enzyme thimet oligopeptidase (TOP), a mammalian enzyme, has a similar substrate specificity to the Pz peptidases but shows a much lower identity (14% at most)	Geobacillus sp.