Literature summary for 3.4.24.15 extracted from

Kawasaki, A.; Nakano, H.; Hosokawa, A.; Nakatsu, T.; Kato, H.; Watanabe, K.
The exquisite structure and reaction mechanism of bacterial Pz-peptidase A toward collagenous peptides: X-ray crystallographic structure analysis of PZ-peptidase a reveals differences from mammalian thimet oligopeptidase (2010), J. Biol. Chem., 285, 34972-34980.

Search for more literature for 3.4.24.15

Cloned(Commentary)

Cloned (Comment)	Organism
for crystallization, recombinant Pz-peptidase A is purified from an Escherichia coli strain BL21	Geobacillus sp.

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystallization of recombinant Pz-peptidase A in complex with two phosphinic peptide inhibitors (PPIs) and determination of the crystal structure of these complexes at 1.80-2.00 A resolution is reported. Structure of Pz-peptidase A is closed and globular. It is suggested that collagenous peptide substrates enter the tunnel at the top gateway of the closed Pz-peptidase A molecule, and reactant peptides are released from the bottom gateway after cleavage at the active site located in the center of the tunnel	Geobacillus sp.

Crystallization (Comment)

Organism

crystallization of recombinant Pz-peptidase A in complex with two phosphinic peptide inhibitors (PPIs) and determination of the crystal structure of these complexes at 1.80-2.00 A resolution is reported. Structure of Pz-peptidase A is closed and globular. It is suggested that collagenous peptide substrates enter the tunnel at the top gateway of the closed Pz-peptidase A molecule, and reactant peptides are released from the bottom gateway after cleavage at the active site located in the center of the tunnel

Geobacillus sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
CBZ-Phe-PSI[PO2CH2]-Ala-Lys-Ser	phosphinic peptide inhibitor	Geobacillus sp.
Gly-Pro-Phe-PSI[PO2CH2]-Gly-Pro-Nle	phosphinic peptide inhibitor	Geobacillus sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0082	-	7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl)	pH and temperature not specified in the publication	Geobacillus sp.

Organism

Organism	UniProt	Comment	Textmining
Geobacillus sp.	Q4W803	-	-
Geobacillus sp. MO-1	Q4W803	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl) + H2O	-	Geobacillus sp.	?	-	?
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl) + H2O	-	Geobacillus sp. MO-1	?	-	?

Synonyms

Synonyms	Comment	Organism
Pz-peptidase A	-	Geobacillus sp.

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000088	-	Gly-Pro-Phe-PSI[PO2CH2]-Gly-Pro-Nle	pH and temperature not specified in the publication	Geobacillus sp.
0.00009	-	CBZ-Phe-PSI[PO2CH2]-Ala-Lys-Ser	pH and temperature not specified in the publication	Geobacillus sp.