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Literature summary for 3.4.24.15 extracted from

  • Sigman, J.A.; Patwa, T.H.; Tablante, A.V.; Joseph, C.D.; Glucksman, M.J.; Wolfson, A.J.
    Flexibility in substrate recognition by thimet oligopeptidase as revealed by denaturation studies (2005), Biochem. J., 388, 255-261.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.15

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000057
-
 7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin pH 7.8, 23°C Rattus norvegicus
0.00013
-
 7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin pH 7.8, 23°C, presence of 1.5 M urea Rattus norvegicus
0.00048
-
 7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin pH 7.8, 23°C, presence of 2.5 M urea Rattus norvegicus
0.00127
-
 7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin pH 7.8, 23°C, presence of 3.5 M urea Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ second step of structural unfolding results in complete loss of catalytic Zn2+ Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P24155
-
-

Renatured (Commentary)

Renatured (Comment) Organism
thermal unfolding is a two-step process with a major endotherm at a Tm of 64°C and a minor endotherm at a Tm of 55°C Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin + H2O
-
 Rattus norvegicus ?
-
 ?
additional information conformational changes due to chemical denaturation are directly correlated with a change in substrate specificity Rattus norvegicus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin pH 7.8, 23°C Rattus norvegicus
0.72
-
 7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin pH 7.8, 23°C, presence of 1.5 M urea Rattus norvegicus
0.91
-
 7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin pH 7.8, 23°C, presence of 2.5 M urea Rattus norvegicus
1.07
-
 7-methoxycoumarin-4-acetyl-(L-Ala-L-Lys(2,4-dinitrophenyl))-bradykinin pH 7.8, 23°C, presence of 3.5 M urea Rattus norvegicus