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Literature summary for 3.4.23.46 extracted from

  • Mancini, F.; Andrisano, V.
    Development of a liquid chromatographic system with fluorescent detection for beta-secretase immobilized enzyme reactor on-line enzymatic studies (2010), J. Pharm. Biomed. Anal., 52, 355-361.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(-)-gallocatechin gallate less potent inhibitor Homo sapiens
Lys-Thr-Glu-Glu-Ile-Ser-Glu-Val-Asn-[statine(3S,4S)]-Val-Ala-Glu-Phe-OH
-
Homo sapiens
N-((1S,2R)-1-benzyl-3-cyclopropylamino-2-hydroxy-propyl)-5-(methanesulfonyl-methyl-amino)-N'-((R)-1-phenyl-ethyl)-isophthalamide beta-secretase inhibitor IV, potent inhibitor Homo sapiens
OM99-2 i.e. Glu-Val-Asn-[(2R,4S,5S)-5-amino-4-hydroxy-2,7-dimethyl-octanoyl]-Ala-Glu-Phe-OH, potent inhibitor Homo sapiens
pepstatin A less potent inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00191
-
M-2420 peptide apparent value, free enzyme, pH and temperature not specified in the publication Homo sapiens
0.00832
-
M-2420 peptide apparent value, immobilized enzyme reactor enzyme, pH and temperature not specified in the publication Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Swedish mutation of amyloid precursor protein + H2O Homo sapiens
-
fragments of Swedish mutation of amyloid precursor protein
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein-FITC + H2O
-
Homo sapiens ?
-
?
M-2420 peptide + H2O cleavage occurs between Leu and Asp Homo sapiens MCA-Ser-Glu-Val-Asn-Leu + Asp-Ala-Glu-Phe-(N6-dinitrophenylacetyl)Lys-amide
-
?
panvera peptide + H2O
-
Homo sapiens ?
-
?
Swedish mutation of amyloid precursor protein + H2O
-
Homo sapiens fragments of Swedish mutation of amyloid precursor protein
-
?

Synonyms

Synonyms Comment Organism
BACE1
-
Homo sapiens
beta-amyloid precursor protein cleaving enzyme
-
Homo sapiens
beta-secretase
-
Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00004
-
free enzyme, pH and temperature not specified in the publication Homo sapiens OM99-2
0.00014
-
enzyme reactor-immobilized enzyme, pH and temperature not specified in the publication Homo sapiens Lys-Thr-Glu-Glu-Ile-Ser-Glu-Val-Asn-[statine(3S,4S)]-Val-Ala-Glu-Phe-OH
0.00014
-
free enzyme, pH and temperature not specified in the publication Homo sapiens Lys-Thr-Glu-Glu-Ile-Ser-Glu-Val-Asn-[statine(3S,4S)]-Val-Ala-Glu-Phe-OH
0.00046
-
enzyme reactor-immobilized enzyme, pH and temperature not specified in the publication Homo sapiens N-((1S,2R)-1-benzyl-3-cyclopropylamino-2-hydroxy-propyl)-5-(methanesulfonyl-methyl-amino)-N'-((R)-1-phenyl-ethyl)-isophthalamide
0.0007
-
enzyme reactor-immobilized enzyme, pH and temperature not specified in the publication Homo sapiens OM99-2
0.00311
-
free enzyme, pH and temperature not specified in the publication Homo sapiens (-)-gallocatechin gallate
0.02119
-
free enzyme, pH and temperature not specified in the publication Homo sapiens pepstatin A
0.1275
-
enzyme reactor-immobilized enzyme, pH and temperature not specified in the publication Homo sapiens (-)-gallocatechin gallate
0.482
-
enzyme reactor-immobilized enzyme, pH and temperature not specified in the publication Homo sapiens pepstatin A

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.286
-
panvera peptide free enzyme, pH and temperature not specified in the publication Homo sapiens
3.298
-
casein-FITC free enzyme, pH and temperature not specified in the publication Homo sapiens
6.222
-
M-2420 peptide free enzyme, pH and temperature not specified in the publication Homo sapiens