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Literature summary for 3.4.23.46 extracted from

  • Ermolieff, J.; Loy, J.A.; Koelsch, G.; Tang, J.
    Proteolytic activation of recombinant pro-memapsin 2 (pro-beta-secretase) studied with new fluorogenic substrates (2000), Biochemistry, 39, 12450-12456.
    View publication on PubMed
Search for more literature for 3.4.23.46

Inhibitors

Inhibitors Comment Organism Structure
OM99-2
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
 ((7-methoxycoumarin-4-yl)acetyl)-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(2,4-dinitrophenyl) pro-memapsin 2 Homo sapiens
0.0054
-
 Arg-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(4-(4-dimethylaminophenylazo)benzoic acid)-Arg pro-memapsin 2 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50300
-
 x * 50300, SDS-PAGE, pro-memapsin2 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
((7-methoxycoumarin-4-yl)acetyl)-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(2,4-dinitrophenyl) + H2O i.e. FS-2, cleavage occurs at Leu-Asp bond Homo sapiens ((7-methoxycoumarin-4-yl)acetyl)-Ser-Glu-Val-Asn-Leu + Asp-Ala-Glu-Phe-Lys(2,4-dinitrophenyl)
-
 ?
amyloid precursor protein + H2O
-
 Homo sapiens ?
-
 ?
Arg-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(4-(4-dimethylaminophenylazo)benzoic acid)-Arg i.e. FS-1, cleavage occurs at Leu-Asp bond Homo sapiens Arg-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Glu-Val-Asn-Leu + Asp-Ala-Glu-Phe-Lys(4-(4-dimethylaminophenylazo)benzoic acid)-Arg
-
 ?
Swedish variant of amyloid precursor protein + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50300, SDS-PAGE, pro-memapsin2 Homo sapiens
More stable in vitro activated forms have 44100 and 46100 kDa, depending on activating protease Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000098
-
 OM99-2 pro-memapsin 2 Homo sapiens