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Literature summary for 3.4.23.4 extracted from

  • Sun, Q.; Wang, X.P.; Yan, Q.J.; Chen, W.; Jiang, Z.Q.
    Purification and characterization of a chymosin from Rhizopus microsporus var. rhizopodiformis (2014), Appl. Biochem. Biotechnol., 174, 174-185.
    View publication on PubMed
Search for more literature for 3.4.23.4

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ milkclotting activity decrease when the concentration of Ca2+ is higher than 40 mM Rhizopus microsporus
EDTA 10 mM EDTA causes inhibition of 73.8% of the milk-clotting activity Rhizopus microsporus
iodoacetamide 4 mM iodoacetamide causes inhibition of 7.2% of the milk-clotting activity Rhizopus microsporus
additional information phenylmethylsulfonyl fluoride does not affect the enzyme activity Rhizopus microsporus
pepstatin A complete inhibition at 0.02 mM Rhizopus microsporus
Sn2+ 28.8% inhibition at 1 mM Rhizopus microsporus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM increases the milk clotting activity by 21.8% Rhizopus microsporus
Cr3+ 1 mM increases the milk clotting activity by 15.1% Rhizopus microsporus
Mg2+ 1 mM increases the milk clotting activity by 20.1% Rhizopus microsporus
additional information Cu2+, Mn2+, and Co2+ ions have no effect on the enzyme activity Rhizopus microsporus
Zn2+ 1 mM increases the milk clotting activity by 12% Rhizopus microsporus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37000, SDS-PAGE Rhizopus microsporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-casein + H2O Rhizopus microsporus alpha-casein is hydrolyzed extensively ?
-
 ?
alpha-casein + H2O Rhizopus microsporus F518 alpha-casein is hydrolyzed extensively ?
-
 ?
beta-casein + H2O Rhizopus microsporus
-
 ?
-
 ?
beta-casein + H2O Rhizopus microsporus F518
-
 ?
-
 ?
kappa-casein + H2O Rhizopus microsporus
-
 ?
-
 ?
kappa-casein + H2O Rhizopus microsporus F518
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizopus microsporus
-
 variant rhizopodiformis
-
Rhizopus microsporus F518
-
 variant rhizopodiformis
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q Sepharose column chromatography, and Sephadex S-100 gel filtration Rhizopus microsporus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.987
-
 crude enzyme, in 50 mM acetate buffer (pH 5.6) at 37°C Rhizopus microsporus
7.272
-
 after 33.2fold purification, in 50 mM acetate buffer (pH 5.6) at 37°C Rhizopus microsporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-casein + H2O alpha-casein is hydrolyzed extensively Rhizopus microsporus ?
-
 ?
alpha-casein + H2O alpha-casein is hydrolyzed extensively Rhizopus microsporus F518 ?
-
 ?
beta-casein + H2O
-
 Rhizopus microsporus ?
-
 ?
beta-casein + H2O
-
 Rhizopus microsporus F518 ?
-
 ?
kappa-casein + H2O
-
 Rhizopus microsporus ?
-
 ?
kappa-casein + H2O
-
 Rhizopus microsporus F518 ?
-
 ?
skim milk + H2O
-
 Rhizopus microsporus ?
-
 ?
skim milk + H2O
-
 Rhizopus microsporus F518 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Rhizopus microsporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Rhizopus microsporus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 the enzyme is stable until 40°C for 30 min while losing 40 and 80% of its activity after incubation at 45 and 50°C for 30 min, respectively Rhizopus microsporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
 Rhizopus microsporus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 8 the enzyme retains 80% of activity within pH 2.0-8.0 Rhizopus microsporus