Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | milkclotting activity decrease when the concentration of Ca2+ is higher than 40 mM | Rhizopus microsporus | |
EDTA | 10 mM EDTA causes inhibition of 73.8% of the milk-clotting activity | Rhizopus microsporus | |
iodoacetamide | 4 mM iodoacetamide causes inhibition of 7.2% of the milk-clotting activity | Rhizopus microsporus | |
additional information | phenylmethylsulfonyl fluoride does not affect the enzyme activity | Rhizopus microsporus | |
pepstatin A | complete inhibition at 0.02 mM | Rhizopus microsporus | |
Sn2+ | 28.8% inhibition at 1 mM | Rhizopus microsporus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM increases the milk clotting activity by 21.8% | Rhizopus microsporus | |
Cr3+ | 1 mM increases the milk clotting activity by 15.1% | Rhizopus microsporus | |
Mg2+ | 1 mM increases the milk clotting activity by 20.1% | Rhizopus microsporus | |
additional information | Cu2+, Mn2+, and Co2+ ions have no effect on the enzyme activity | Rhizopus microsporus | |
Zn2+ | 1 mM increases the milk clotting activity by 12% | Rhizopus microsporus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Rhizopus microsporus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-casein + H2O | Rhizopus microsporus | alpha-casein is hydrolyzed extensively | ? | - |
? | |
alpha-casein + H2O | Rhizopus microsporus F518 | alpha-casein is hydrolyzed extensively | ? | - |
? | |
beta-casein + H2O | Rhizopus microsporus | - |
? | - |
? | |
beta-casein + H2O | Rhizopus microsporus F518 | - |
? | - |
? | |
kappa-casein + H2O | Rhizopus microsporus | - |
? | - |
? | |
kappa-casein + H2O | Rhizopus microsporus F518 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizopus microsporus | - |
variant rhizopodiformis | - |
Rhizopus microsporus F518 | - |
variant rhizopodiformis | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q Sepharose column chromatography, and Sephadex S-100 gel filtration | Rhizopus microsporus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.987 | - |
crude enzyme, in 50 mM acetate buffer (pH 5.6) at 37°C | Rhizopus microsporus |
7.272 | - |
after 33.2fold purification, in 50 mM acetate buffer (pH 5.6) at 37°C | Rhizopus microsporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-casein + H2O | alpha-casein is hydrolyzed extensively | Rhizopus microsporus | ? | - |
? | |
alpha-casein + H2O | alpha-casein is hydrolyzed extensively | Rhizopus microsporus F518 | ? | - |
? | |
beta-casein + H2O | - |
Rhizopus microsporus | ? | - |
? | |
beta-casein + H2O | - |
Rhizopus microsporus F518 | ? | - |
? | |
kappa-casein + H2O | - |
Rhizopus microsporus | ? | - |
? | |
kappa-casein + H2O | - |
Rhizopus microsporus F518 | ? | - |
? | |
skim milk + H2O | - |
Rhizopus microsporus | ? | - |
? | |
skim milk + H2O | - |
Rhizopus microsporus F518 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Rhizopus microsporus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Rhizopus microsporus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
the enzyme is stable until 40°C for 30 min while losing 40 and 80% of its activity after incubation at 45 and 50°C for 30 min, respectively | Rhizopus microsporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
- |
Rhizopus microsporus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 8 | the enzyme retains 80% of activity within pH 2.0-8.0 | Rhizopus microsporus |