Application | Comment | Organism |
---|---|---|
food industry | the enzyme is used industrially in cheese production | Bos taurus |
food industry | the enzyme is used industrially in cheese production | Camelus dromedarius |
Crystallization (Comment) | Organism |
---|---|
doubly and singly glycosylated variants of chymosin, vapor diffusion method, using 2 M ammonium sulfate, 100 mM bis-Tris buffer in the pH range 5.1-6.5 | Camelus dromedarius |
unglycosylated chymosin, vapor diffusion method, using 100 mM NaH2PO4 pH 5.5, 1.5 M NaCl | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the loss of the first three residues of camel chymosin significantly decreases enzyme activity | Camelus dromedarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-casein + H2O | Bos taurus | the enzyme cleaves the Phe105-Met106 bond of kappa-casein, releasing its predominantly negatively charged C-terminus | ? | - |
? | |
kappa-casein + H2O | Camelus dromedarius | the enzyme cleaves the Phe105-Met106 bond of kappa-casein, releasing its predominantly negatively charged C-terminus | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P00794 | - |
- |
Camelus dromedarius | Q9GK11 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | glycosylation at Asn291 significantly decreases enzyme activity | Bos taurus |
glycoprotein | glycosylation at Asn291 significantly decreases enzyme activity | Camelus dromedarius |
Purification (Comment) | Organism |
---|---|
phenyl Superose column chromatography | Bos taurus |
phenyl Superose column chromatography | Camelus dromedarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-casein + H2O | the enzyme cleaves the Phe105-Met106 bond of kappa-casein, releasing its predominantly negatively charged C-terminus | Bos taurus | ? | - |
? | |
kappa-casein + H2O | the enzyme cleaves the Phe105-Met106 bond of kappa-casein, releasing its predominantly negatively charged C-terminus | Camelus dromedarius | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * about 40000, mass spectrometry | Bos taurus |
? | x * about 40000, mass spectrometry | Camelus dromedarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
melting temperature of the singly glycosylated enzyme | Camelus dromedarius |