Cloned (Comment) | Organism |
---|---|
expression in Kluyveromyces lactis | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pepstatin | inhibits recombinant and transgenic chymosin less efficiently than the native enzyme | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | transgenic and recombinant chymosin do not hydrolyze substrate Abz-A-A-F-F-A-A-p-nitroanilide, Abz-A-A-F-F-N-(2,4-dinitrophenyl)-ethylenediamine and Abz-A-A-F-F-p-nitroanilide | Bos taurus | |
0.0018 | - |
Abz-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by calf chymosin | Bos taurus | |
0.0018 | - |
Abz-A-A-F-F-p-nitroanilide | hydrolysis by calf chymosin | Bos taurus | |
0.0021 | - |
Abz-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by recombinant chymosin | Bos taurus | |
0.0022 | - |
Abz-A-A-F-F-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by calf chymosin | Bos taurus | |
0.003 | - |
Abz-A-A-F-F-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by calf chymosin | Bos taurus | |
0.0035 | - |
Abz-A-A-F-F-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by recombinant chymosin | Bos taurus | |
0.004 | - |
Abz-A-A-F-F-A-A-p-nitroanilide | hydrolysis by calf chymosin | Bos taurus | |
0.0056 | - |
Abz-A-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by recombinant chymosin | Bos taurus | |
0.0056 | - |
Abz-A-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by transgenic chymosin | Bos taurus | |
0.007 | - |
Abz-A-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by calf chymosin | Bos taurus | |
0.0078 | - |
Abz-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by transgenic chymosin | Bos taurus | |
0.018 | - |
Abz-A-A-F-F-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by transgenic chymosin | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by affinity chromatography on a bacitracin-Sepharose column | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
abomasum | - |
Bos taurus | - |
milk | transgenic sheep milk | Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-A-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine + H2O | low molecular weight, fluorogenic peptide substrate | Bos taurus | ? | - |
? | |
Abz-A-A-F-F-A-A-p-nitroanilide + H2O | low molecular weight, fluorogenic peptide substrate | Bos taurus | ? | - |
? | |
Abz-A-A-F-F-A-N-(2,4-dinitrophenyl)-ethylenediamine + H2O | low molecular weight, fluorogenic peptide substrate | Bos taurus | ? | - |
? | |
Abz-A-A-F-F-N-(2,4-dinitrophenyl)-ethylenediamine + H2O | low molecular weight, fluorogenic peptide substrate | Bos taurus | ? | - |
? | |
Abz-A-A-F-F-pnA + H2O | low molecular weight, fluorogenic peptide substrate | Bos taurus | ? | - |
? | |
Abz-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine + H2O | low molecular weight, fluorogenic peptide substrate | Bos taurus | ? | - |
? | |
L-S-F-M-A-I-P-NH2 + H2O | hepapeptide, a fragment of the native chymosin substrate kappa-casein, is most efficiently cleaved by native calf chymosin and less efficiently by transgenic chymosin and recombinant chymosin | Bos taurus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | transgenic chymosin loses activity slightly faster than abomasal enzyme and recombinant enzyme | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
Abz-A-A-F-F-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by transgenic chymosin | Bos taurus | |
0.06 | - |
Abz-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by transgenic chymosin or recombinat chymosin | Bos taurus | |
0.11 | - |
Abz-A-A-F-F-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by recombinant chymosin | Bos taurus | |
0.12 | - |
Abz-A-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by recombinant chymosin | Bos taurus | |
0.14 | - |
Abz-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by calf chymosin | Bos taurus | |
0.16 | - |
Abz-A-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by transgenic chymosin | Bos taurus | |
0.27 | - |
Abz-A-A-F-F-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by calf chymosin | Bos taurus | |
0.48 | - |
Abz-A-A-F-F-p-nitroanilide | hydrolysis by calf chymosin | Bos taurus | |
0.8 | - |
Abz-A-A-F-F-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by calf chymosin | Bos taurus | |
1.4 | - |
Abz-A-A-F-F-A-A-p-nitroanilide | hydrolysis by calf chymosin | Bos taurus | |
1.6 | - |
Abz-A-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine | hydrolysis by calf chymosin | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 6 | under incubation at 37°C for 24 and 48 h, loss of activity at pH 3.5 and pH 7.0, trangenic chymosin and recombinant chymosin are inactivated slightly faster than the native enzyme | Bos taurus |