Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.4 extracted from

  • Kageyama, T.
    Role of S1 loop residues in the substrate specificities of pepsin A and chymosin (2004), Biochemistry, 43, 15122-15130.
    View publication on PubMed
Search for more literature for 3.4.23.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae strain INVSC1 Callithrix jacchus

Protein Variants

Protein Variants Comment Organism
D289M/Q298L decreased hydrolytic activity Callithrix jacchus
additional information Asp289Met mutant, Gln298Leu mutant, hydrolytic activity toward peptides having Lys at P'1 decreases significantly, mainly because of a decrease in the turnover values Callithrix jacchus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage L147-F148) by D289M/Q298L mutant chymosin Callithrix jacchus
0.024
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage F147-R148) by D289M/Q298L mutant chymosin Callithrix jacchus
0.028
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage F147-R148) by Asp289Met mutant chymosin Callithrix jacchus
0.029
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage L147-F148) by Gln298Leu mutant chymosin Callithrix jacchus
0.033
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage F147-R148) by Gln298Leu mutant chymosin Callithrix jacchus
0.046
-
 NT/NMN 142-151 hydrolysis of the Glu148 variant by D289M/Q298L mutant chymosin Callithrix jacchus
0.054
-
 NT/NMN 142-151 hydrolysis of the Glu148 variant by Asp289Met mutant chymosin Callithrix jacchus
0.054
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage L147-F148) by Asp289Met mutant chymosin Callithrix jacchus
0.059
-
 NT/NMN 142-151 hydrolysis of the parent peptide by wild type chymosin Callithrix jacchus
0.062
-
 NT/NMN 142-151 hydrolysis of the Glu148 variant by Gln298Leu mutant chymosin Callithrix jacchus
0.065
-
 POm C165-174 hydrolysis of the Glu171 variant by Asp289Met mutant chymosin Callithrix jacchus
0.072
-
 NT/NMN 142-151 hydrolysis of the parent peptide by Asp289Met mutant chymosin Callithrix jacchus
0.083
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage L147-F148) by wild type chymosin Callithrix jacchus
0.087
-
 NT/NMN 142-151 hydrolysis of the parent peptide by D289M/Q298L mutant chymosin Callithrix jacchus
0.095
-
 NT/NMN 142-151 hydrolysis of the parent peptide by Gln298Leu mutant chymosin Callithrix jacchus
0.105
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage F147-R148) by wild type chymosin Callithrix jacchus
0.111
-
 NT/NMN 142-151 hydrolysis of the Glu148 variant by wild type chymosin Callithrix jacchus
0.114
-
 POm C165-174 hydrolysis of the Glu171 variant by D289M/Q298L mutant chymosin Callithrix jacchus
0.229
-
 POm C165-174 hydrolysis of the parent peptide by Gln298Leu mutant chymosin Callithrix jacchus
0.25
-
 POm C165-174 hydrolysis of the parent peptide by D289M/Q298L mutant chymosin Callithrix jacchus
0.288
-
 POm C165-174 hydrolysis of the parent peptide by Asp289Met mutant chymosin Callithrix jacchus
0.304
-
 POm C165-174 hydrolysis of the Glu171 variant by wild type chymosin Callithrix jacchus
0.375
-
 POm C165-174 hydrolysis of the parent peptide by wild type chymosin Callithrix jacchus
0.463
-
 POm C165-174 hydrolysis of the Glu171 variant by Gln298Leu mutant chymosin Callithrix jacchus

Organism

Organism UniProt Comment Textmining
Callithrix jacchus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Callithrix jacchus

Storage Stability

Storage Stability Organism
-20°C, Tris-HCl buffer, pH 8.0, glycerol Callithrix jacchus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
basic FGF 110-118 + H2O parent peptide and Leu115 and Lys115 variants respectively as substrate Callithrix jacchus ?
-
 ?
dynorphin A 1-7e + H2O Ala3Phe7 and ILe3Lys5Phe7 variants respectively as substrate Callithrix jacchus ?
-
 ?
NT/NMN 142-151 + H2O parent peptide, Glu148 and Phe148 variants respectively as substrate Callithrix jacchus ?
-
 ?
POm C165-174 + H2O parent peptide and Glu171 variant respectively as substrate Callithrix jacchus ?
-
 ?
Substance P + H2O parent peptide and Lys8 variant respectively as substrate Callithrix jacchus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage L147-F148) by wild type chymosin Callithrix jacchus
12
-
 POm C165-174 hydrolysis of the Glu171 variant by D289M/Q298L mutant chymosin Callithrix jacchus
12
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage L147-F148) by Gln298Leu mutant chymosin Callithrix jacchus
14
-
 POm C165-174 hydrolysis of the Glu171 variant by Asp289Met mutant chymosin Callithrix jacchus
16
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage L147-F148) by D289M/Q298L mutant chymosin Callithrix jacchus
17
-
 NT/NMN 142-151 hydrolysis of the Glu148 variant by Gln298Leu mutant chymosin Callithrix jacchus
17
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage F147-R148) by D289M/Q298L mutant chymosin Callithrix jacchus
22
-
 NT/NMN 142-151 hydrolysis of the Glu148 variant by D289M/Q298L mutant chymosin Callithrix jacchus
27
-
 NT/NMN 142-151 hydrolysis of the parent peptide by D289M/Q298L mutant chymosin Callithrix jacchus
30
-
 NT/NMN 142-151 hydrolysis of the parent peptide by Gln298Leu mutant chymosin Callithrix jacchus
32
-
 NT/NMN 142-151 hydrolysis of the Glu148 variant by Asp289Met mutant chymosin Callithrix jacchus
35
-
 NT/NMN 142-151 hydrolysis of the parent peptide by Asp289Met mutant chymosin Callithrix jacchus
39
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage L147-F148) by Asp289Met mutant chymosin Callithrix jacchus
41
-
 NT/NMN 142-151 hydrolysis of the parent peptide by wild type chymosin Callithrix jacchus
42
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage F147-R148) by Asp289Met mutant chymosin Callithrix jacchus
43
-
 POm C165-174 hydrolysis of the parent peptide by D289M/Q298L mutant chymosin Callithrix jacchus
46
-
 NT/NMN 142-151 hydrolysis of the Glu148 variant by wild type chymosin Callithrix jacchus
49
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage F147-R148) by Gln298Leu mutant chymosin Callithrix jacchus
52
-
 POm C165-174 hydrolysis of the Glu171 variant by Gln298Leu mutant chymosin Callithrix jacchus
63
-
 POm C165-174 hydrolysis of the Glu171 variant by wild type chymosin Callithrix jacchus
90
-
 POm C165-174 hydrolysis of the parent peptide by Gln298Leu mutant chymosin Callithrix jacchus
101
-
 POm C165-174 hydrolysis of the parent peptide by Asp289Met mutant chymosin Callithrix jacchus
191
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage F147-R148) by wild type chymosin Callithrix jacchus
219
-
 POm C165-174 hydrolysis of the parent peptide by wild type chymosin Callithrix jacchus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Callithrix jacchus