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Literature summary for 3.4.23.24 extracted from

  • Aoki, W.; Kitahara, N.; Miura, N.; Morisaka, H.; Yamamoto, Y.; Kuroda, K.; Ueda, M.
    Comprehensive characterization of secreted aspartic proteases encoded by a virulence gene family in Candida albicans (2011), J. Biochem., 150, 431-438.
    View publication on PubMed
Search for more literature for 3.4.23.24

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
additional information isozyme Sap7 is not inhibited by pepstatin A, leupeptin, phenylmethylsulfonyl fluoride and EDTA Candida albicans
pepstatin A isozymes 1-10 except 7 Candida albicans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11000
-
 1 * 44000 + 1 * 11000, isozyme Sap9, SDS-PAGE Candida albicans
15000
-
 1 * 52000 + 1 * 15000, isozyme Sap7, SDS-PAGE Candida albicans
35000
-
 isozyme Sap8, calculated from amino acid sequence Candida albicans
36000
-
 isozyme Sap1, calculated from amino acid sequence Candida albicans
36000
-
 isozyme Sap2, calculated from amino acid sequence Candida albicans
36000
-
 isozyme Sap5, estimated from SDS-PAGE Candida albicans
36000
-
 isozyme Sap8, estimated from SDS-PAGE Candida albicans
37000
-
 isozyme Sap3, SDS-PAGE and calculated from amino acid sequence Candida albicans
37000
-
 isozyme Sap4, calculated from amino acid sequence Candida albicans
37000
-
 isozyme Sap5, calculated from amino acid sequence Candida albicans
37000
-
 isozyme Sap6, calculated from amino acid sequence Candida albicans
38000
-
 isozyme Sap2, estimated from SDS-PAGE Candida albicans
38000
-
 isozyme Sap6, estimated from SDS-PAGE Candida albicans
39000
-
 isozyme Sap1, estimated from SDS-PAGE Candida albicans
39000
-
 isozyme Sap4, estimated from SDS-PAGE Candida albicans
42000
-
 isozyme Sap10, estimated from SDS-PAGE Candida albicans
44000
-
 isozyme Sap9, estimated from SDS-PAGE Candida albicans
44000
-
 1 * 44000 + 1 * 11000, isozyme Sap9, SDS-PAGE Candida albicans
45000
-
 isozyme Sap10, calculated from amino acid sequence Candida albicans
47000
-
 isozyme Sap7, calculated from amino acid sequence Candida albicans
52000
-
 isozyme Sap7, estimated from SDS-PAGE Candida albicans
52000
-
 1 * 52000 + 1 * 15000, isozyme Sap7, SDS-PAGE Candida albicans
53000
-
 isozyme Sap9, calculated from amino acid sequence Candida albicans

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-
Candida albicans P0CY27
-
-
Candida albicans P0CY29
-
-
Candida albicans P0DJ06
-
-
Candida albicans P43094
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein isozymes Sap4, 6, 8, 9 and 10 are N-glycosylated. Sap7 occurs in glycosylated forms other than N-glycosylation Candida albicans

Purification (Commentary)

Purification (Comment) Organism
anti-FLAG M2 affinity gel filtration Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O isozyme Sap6 shows highest activity towards casein Candida albicans ?
-
 ?
FRETS-25Ala + H2O isozyme Sap1 shows highest activity towards FRETS-25Ala Candida albicans ?
-
 ?
FRETS-25Xaa + H2O all Sap isozymes prefer Arg and Lys at the Xaa position Candida albicans ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 44000 + 1 * 11000, isozyme Sap9, SDS-PAGE Candida albicans
heterodimer 1 * 52000 + 1 * 15000, isozyme Sap7, SDS-PAGE Candida albicans

Synonyms

Synonyms Comment Organism
SAP
-
 Candida albicans
SAP1 isozyme Candida albicans
Sap10 isozyme Candida albicans
SAP2 isozyme Candida albicans
SAP3 isozyme Candida albicans
SAP4 isozyme Candida albicans
SAP5 isozyme Candida albicans
SAP6 isozyme Candida albicans
Sap7 isozyme Candida albicans
Sap8 isozyme Candida albicans
Sap9 isozyme Candida albicans
secreted aspartic protease
-
 Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
 isozyme Sap8 Candida albicans
3
-
 isozyme Sap3 Candida albicans
4
-
 isozyme Sap2 Candida albicans
5
-
 isozyme Sap1 Candida albicans
5
-
 isozyme Sap4 Candida albicans
5
-
 isozyme Sap5 Candida albicans
5
-
 isozyme Sap6 Candida albicans
5.5
-
 isozyme Sap9 Candida albicans
6
-
 isozyme Sap10 Candida albicans
6.5
-
 isozyme Sap7 Candida albicans