Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.16 extracted from

  • Hong, L.; Hartsuck, J.A.; Foundling, S.; Ermolieff, J.; Tang, J.
    Active-site mobility in human immunodeficiency virus, type 1, protease as demonstrated by crystal structure of A28S mutant (1998), Protein Sci., 7, 300-305.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.16

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method at 20°C, A28S mutant enzyme in complex with the peptide inhibitor U-89360E Human immunodeficiency virus 1

Protein Variants

Protein Variants Comment Organism
A28S the ratio of turnover number to Km-value for the substrate TATIMMQRGN is 1527fold lower than that for the wild-type enzyme. The ratio of turnover number to Km-value for the substrate RQGTVSFNFPQITL is 1488fold lower than that for the wild-type enzyme Human immunodeficiency virus 1

Inhibitors

Inhibitors Comment Organism Structure
U-89360E
-
 Human immunodeficiency virus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 TATIMMQRGN pH 5.0, 37°C, mutant enzyme A28S Human immunodeficiency virus 1
0.34
-
 RQGTVSFNFPQITL pH 5.0, 37°C, mutant enzyme A28S Human immunodeficiency virus 1
0.54
-
 RQGTVSFNFPQITL pH 5.0, 37°C, wild-type enzyme Human immunodeficiency virus 1

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Human immunodeficiency virus 1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RQGTVSFNFPQITL + H2O
-
 Human immunodeficiency virus 1 RQGTVSFNF + PQITL
-
 ?
TATIMMQRGN + H2O
-
 Human immunodeficiency virus 1 TATIM + MQRGN
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.018
-
 TATIMMQRGN pH 5.0, 37°C, mutant enzyme A28S Human immunodeficiency virus 1
0.026
-
 RQGTVSFNFPQITL pH 5.0, 37°C, mutant enzyme A28S Human immunodeficiency virus 1
58.8
-
 TATIMMQRGN pH 5.0, 37°C, wild-type enzyme Human immunodeficiency virus 1
62.7
-
 RQGTVSFNFPQITL pH 5.0, 37°C, wild-type enzyme Human immunodeficiency virus 1