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Literature summary for 3.4.22.56 extracted from

  • Valanne, A.; Malmi, P.; Appelblom, H.; Niemelae, P.; Soukka, T.
    A dual-step fluorescence resonance energy transfer-based quenching assay for screening of caspase-3 inhibitors (2008), Anal. Biochem., 375, 71-81.
    View publication on PubMed
Search for more literature for 3.4.22.56

Inhibitors

Inhibitors Comment Organism Structure
carbobenzyloxy-DEVD-fluoromethyl ketone a caspase-3 inhibitor Homo sapiens
additional information screening of caspase-3 inhibitors using a dual-step fluorescence resonance energy transfer-based quenching assay for, method development with fluorescent europium(III)-chelate-doped nanoparticle donors coated with streptavidin in conjunction with a dual-labeled caspase-3-specific peptide substrate modified with a biotinyl moiety, overview Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation recombinant caspase-3 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CDEVDK + H2O
-
 Homo sapiens acetyl-CDEVD + Lys
-
 ?
biotinylated acetyl-CDEVDK + H2O
-
 Homo sapiens biotinylated acetyl-CDEVD + Lys
-
 ?
additional information the aspartic acid residue in position P1 is a nearly absolute requirement for the caspase activity, at least three additional amino acids at positions P2 to P4 are required to complete the specificity Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Homo sapiens