Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dimeric calpain I requires 0.002 mM for half-maximal activation and 0.01 mM for maximal activation. The 70000 Da monomeric calpain I requires only 0.001 mM Ca2+ for half-maximal activity | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
1 * 83000 + 1 * 29000, an isolated 70000 Da calpain I, or monomeric artifact is also detected, SDS-PAGE | Sus scrofa |
83000 | - |
1 * 83000 + 1 * 29000, an isolated 70000 Da calpain I, or monomeric artifact is also detected, SDS-PAGE | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
polymorphonuclear leukocyte | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 83000 + 1 * 29000, an isolated 70000 Da calpain I, or monomeric artifact is also detected, SDS-PAGE | Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the 70000 Da monomeric calpain I is much less heat stable than the parent heterodimeric calpain I | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
monomeric calpain I | Sus scrofa |
7.5 | - |
dimeric calpain I | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | pH 6.5: about 35% of maximal activity, pH 8.0: about 50% of maximal activity | Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the 70000 Da monomeric calpain I is less pH stable than the parent heterodimeric calpain I | Sus scrofa |