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Literature summary for 3.4.22.36 extracted from

  • Luksch, H.; Romanowski, M.J.; Chara, O.; Tuengler, V.; Caffarena, E.R.; Heymann, M.C.; Lohse, P.; Aksentijevich, I.; Remmers, E.F.; Flecks, S.; Quoos, N.; Gramatte, J.; Petzold, C.; Hofmann, S.R.; Winkler, S.; Pessler, F.; Kallinich, T.; Ganser, G.; Nimtz-Talaska, A.; Baumann, U.; Runde, V.; Grimbacher, B., et al
    Naturally occurring genetic variants of human caspase-1 differ considerably in structure and the ability to activate interleukin-1beta (2013), Hum. Mutat., 34, 122-131.
    View publication on PubMed
Search for more literature for 3.4.22.36

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Codon Plus cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme mutants in complex with 3-[2-(2-benzyloxycarbonylamino-3-methyl-butyrylamino)-propionylamino]-4-oxo-pentanoic acid, hanging drop vapor diffusion method, using 0.1 M PIPES pH 6.0, 75-175 mM (NH4)2SO4, 25% (w/v) PEG 2000 MME, 10 mM dithiothreitol, 3 mM NaN3, and 2 mM MgCl2 Homo sapiens

Protein Variants

Protein Variants Comment Organism
A329T the mutant demonstrates absent enzymatic and interleukin-1beta releasing activity in vitro Homo sapiens
C285A inactive Homo sapiens
K319R the mutant demonstrates decreased enzymatic and interleukin-1beta releasing activity in vitro Homo sapiens
L265S the mutant demonstrates absent enzymatic and interleukin-1beta releasing activity in vitro Homo sapiens
N263S the mutant demonstrates decreased enzymatic and interleukin-1beta releasing activity in vitro Homo sapiens
R221C the mutant demonstrates absent enzymatic and interleukin-1beta releasing activity in vitro Homo sapiens
R240Q the mutant demonstrates decreased enzymatic and interleukin-1beta releasing activity in vitro Homo sapiens
T267I the mutant demonstrates absent enzymatic and interleukin-1beta releasing activity in vitro Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10244
-
 1 * 19844 + 1 * 10244, calculated from amino acid sequence Homo sapiens
19844
-
 1 * 19844 + 1 * 10244, calculated from amino acid sequence Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pro-interleukin-1beta + H2O Homo sapiens
-
 interleukin-1beta propeptide
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P29466
-
-

Source Tissue

Source Tissue Comment Organism Textmining
THP-1 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-WEHD-7-amido-4-trifluoromethylcoumarin + H2O
-
 Homo sapiens N-acetyl-WEHD + 7-amino-4-trifluoromethylcoumarin
-
 ?
pro-interleukin-1beta + H2O
-
 Homo sapiens interleukin-1beta propeptide
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 19844 + 1 * 10244, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
Casp1
-
 Homo sapiens
ICE
-
 Homo sapiens
interleukin-1 converting enzyme
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction decreased enzymatic activity of caspase-1 is compatible with normal life and does not prevent moderate and severe autoinflammation Homo sapiens
physiological function caspase-1 is a proinflammatory enzyme that plays pivotal roles in innate immunity and many inflammatory conditions such as periodic fever syndromes and gout Homo sapiens