Application | Comment | Organism |
---|---|---|
food industry | the immobilized stem bromelain has productive biotechnological applications in wine-making | Ananas comosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethanol | competitive inhibition | Ananas comosus | |
additional information | grape skin and seed tannins exert uncompetitive inhibition | Ananas comosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03919 | - |
Bz-Phe-Val-Arg-4-nitroanilide | chitosan-immobilized enzyme, at pH 3.2 and 25°C | Ananas comosus | |
0.0495 | - |
Bz-Phe-Val-Arg-4-nitroanilide | chitosan-immobilized enzyme, in the presence of 12% (v/v) ethanol, at pH 3.2 and 25°C | Ananas comosus | |
0.05731 | - |
Bz-Phe-Val-Arg-4-nitroanilide | chitosan-immobilized enzyme, in the presence of 18% (v/v) ethanol, at pH 3.2 and 25°C | Ananas comosus | |
0.118 | - |
Bz-Phe-Val-Arg-4-nitroanilide | free enzyme, at pH 3.2 and 25°C | Ananas comosus | |
0.25 | - |
Bz-Phe-Val-Arg-4-nitroanilide | free enzyme, in the presence of 12% (v/v) ethanol, at pH 3.2 and 25°C | Ananas comosus | |
0.305 | - |
Bz-Phe-Val-Arg-4-nitroanilide | free enzyme, in the presence of 18% (v/v) ethanol, at pH 3.2 and 25°C | Ananas comosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ananas comosus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | - |
Ananas comosus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bz-Phe-Val-Arg-4-nitroanilide + H2O | - |
Ananas comosus | Bz-Phe-Val-Arg + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bromelain | - |
Ananas comosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
Bz-Phe-Val-Arg-4-nitroanilide | chitosan-immobilized enzyme, in the presence of 12% (v/v) ethanol, at pH 3.2 and 25°C | Ananas comosus | |
0.399 | - |
Bz-Phe-Val-Arg-4-nitroanilide | chitosan-immobilized enzyme, in the presence of 18% (v/v) ethanol, at pH 3.2 and 25°C | Ananas comosus | |
0.461 | - |
Bz-Phe-Val-Arg-4-nitroanilide | chitosan-immobilized enzyme, at pH 3.2 and 25°C | Ananas comosus | |
19.44 | - |
Bz-Phe-Val-Arg-4-nitroanilide | free enzyme, at pH 3.2 and 25°C | Ananas comosus | |
20.03 | - |
Bz-Phe-Val-Arg-4-nitroanilide | free enzyme, in the presence of 12% (v/v) ethanol, at pH 3.2 and 25°C | Ananas comosus | |
21.45 | - |
Bz-Phe-Val-Arg-4-nitroanilide | free enzyme, in the presence of 18% (v/v) ethanol, at pH 3.2 and 25°C | Ananas comosus |