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Literature summary for 3.4.22.32 extracted from

  • Esti, M.; Benucci, I.; Liburdi, K.; Garzillo, A.
    Immobilized pineapple stem bromelain activity in a wine-like medium: Effect of inhibitors (2015), Food Bioprod. Process., 93, 84-89.
No PubMed abstract available

Application

Application Comment Organism
food industry the immobilized stem bromelain has productive biotechnological applications in wine-making Ananas comosus

Inhibitors

Inhibitors Comment Organism Structure
ethanol competitive inhibition Ananas comosus
additional information grape skin and seed tannins exert uncompetitive inhibition Ananas comosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03919
-
Bz-Phe-Val-Arg-4-nitroanilide chitosan-immobilized enzyme, at pH 3.2 and 25°C Ananas comosus
0.0495
-
Bz-Phe-Val-Arg-4-nitroanilide chitosan-immobilized enzyme, in the presence of 12% (v/v) ethanol, at pH 3.2 and 25°C Ananas comosus
0.05731
-
Bz-Phe-Val-Arg-4-nitroanilide chitosan-immobilized enzyme, in the presence of 18% (v/v) ethanol, at pH 3.2 and 25°C Ananas comosus
0.118
-
Bz-Phe-Val-Arg-4-nitroanilide free enzyme, at pH 3.2 and 25°C Ananas comosus
0.25
-
Bz-Phe-Val-Arg-4-nitroanilide free enzyme, in the presence of 12% (v/v) ethanol, at pH 3.2 and 25°C Ananas comosus
0.305
-
Bz-Phe-Val-Arg-4-nitroanilide free enzyme, in the presence of 18% (v/v) ethanol, at pH 3.2 and 25°C Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
Ananas comosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Bz-Phe-Val-Arg-4-nitroanilide + H2O
-
Ananas comosus Bz-Phe-Val-Arg + 4-nitroaniline
-
?

Synonyms

Synonyms Comment Organism
Bromelain
-
Ananas comosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
Bz-Phe-Val-Arg-4-nitroanilide chitosan-immobilized enzyme, in the presence of 12% (v/v) ethanol, at pH 3.2 and 25°C Ananas comosus
0.399
-
Bz-Phe-Val-Arg-4-nitroanilide chitosan-immobilized enzyme, in the presence of 18% (v/v) ethanol, at pH 3.2 and 25°C Ananas comosus
0.461
-
Bz-Phe-Val-Arg-4-nitroanilide chitosan-immobilized enzyme, at pH 3.2 and 25°C Ananas comosus
19.44
-
Bz-Phe-Val-Arg-4-nitroanilide free enzyme, at pH 3.2 and 25°C Ananas comosus
20.03
-
Bz-Phe-Val-Arg-4-nitroanilide free enzyme, in the presence of 12% (v/v) ethanol, at pH 3.2 and 25°C Ananas comosus
21.45
-
Bz-Phe-Val-Arg-4-nitroanilide free enzyme, in the presence of 18% (v/v) ethanol, at pH 3.2 and 25°C Ananas comosus