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Literature summary for 3.4.22.32 extracted from

  • Dave, S.; Mahajan, S.; Chandra, V.; Gupta, P.
    Trifluoroethanol stabilizes the molten globule state and induces non-amyloidic turbidity in stem bromelain near its isoelectric point (2011), Int. J. Biol. Macromol., 49, 536-542.
    View publication on PubMed

Organic Solvent Stability

Organic Solvent Comment Organism
2,2,2-trifluoroethanol the enhanced binding of 1-anilino-8-naphthalene sulfonic acid to the specific/pre-molten globule (SMG) state of stem bromelain upon addition of 30% 2,2,2-trifluoroethanol suggests the presence of a large number of solvent-accessible non-polar clusters in the treated SMG. The formation of an molten globe (MG)-like state characterized by disordered side chain interactions but with considerable secondary structure when the specific/pre-molten globule (SMG) state of stem bromelain is subjected to 30% 2,2,2-trifluoroethanol (TFE). The TFE-induced MG conformation at alkaline pH could represent the conformation that allows stem bromelain to traverse membranes Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Ananas comosus ?
-
?

Synonyms

Synonyms Comment Organism
Sbm
-
Ananas comosus
stem bromelain
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Ananas comosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Ananas comosus