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BRENDA support

Literature summary for 3.4.22.32 extracted from

  • Gupta, P.; Maqbool, T.; Saleemuddin, M.
    Oriented immobilization of stem bromelain via the lone histidine on a metal affinity support (2007), J. Mol. Catal. B, 45, 78-83.
No PubMed abstract available

General Stability

General Stability Organism
bromelain immobilized to IDA-Sepharose 6B matrix loaded with Cu2+, Ni2+ and Zn2+ is more resistant to thermal inactivation, as evidenced by retention of over 50% activity after incubation at 60°C Ananas comosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23800
-
SDS-PAGE Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
Ananas comosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Ananas comosus ?
-
?

Synonyms

Synonyms Comment Organism
Bromelain
-
Ananas comosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
the native enzyme retains 20% of the initial activity after incubation at 60°C Ananas comosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
immobilized enzyme Ananas comosus
7.5
-
native enzyme Ananas comosus