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Literature summary for 3.4.22.32 extracted from

  • Ahmad, B.; Khan, R.H.
    Studies on the acid unfolded and molten globule states of catalytically active stem bromelain: A comparison with catalytically inactive form (2006), J. Biochem., 140, 501-508.
    View publication on PubMed

Organic Solvent Stability

Organic Solvent Comment Organism
guanidine-HCl 6 M guanidine-HCl denatured state of stem bromelain is enzymatically inactive Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
Ananas comosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Ananas comosus ?
-
?

pH Stability

pH Stability pH Stability Maximum Comment Organism
0.8 2 stem bromelain at pH 2.0 is maximally unfolded and characterized by significant loss of secondary structure (about 80%) and almost complete loss of tertiary contacts, at pH 0.8 a molten globule state is observed with secondary structure content similar to that of native protein but no tertiary structure Ananas comosus