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Literature summary for 3.4.22.32 extracted from
- Multiple molecular forms of stem bromelain. Isolation and characterization of two closely related components, SB1 and SB2 (1973), J. Biochem., 74, 355-373.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 190 | - |
benzoyl-L-Arg ethyl ester | enzyme form SB1 | Ananas comosus |  |
| 200 | - |
benzoyl-L-Arg ethyl ester | enzyme form SB2, benzoyl-Gly ethyl ester, enzyme form SB1 and SB2 | Ananas comosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25600 | - |
x * 25600, Ananas comosus, enzyme forms SB1 and SB2, SDS-PAGE | Ananas comosus |
| 28000 | - |
Ananas comosus, enzyme form SB1 and SB2, sedimentation equilibrium method | Ananas comosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ananas comosus | - |
2 forms: SB1 and SB2 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate composition: molar ratio of mannose:xylose:fucose is 2.43:1.0:1.01 (enzyme form SB1), 2.55:1.17:0.92 (enzyme form SB2) | Ananas comosus |
| glycoprotein | neutral sugar content: 4.89% (enzyme form SB1), 5.24% (enzyme form SB2) | Ananas comosus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 forms: SB1 and SB2 | Ananas comosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| stem | - |
Ananas comosus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzoyl-Gly ethyl ester + H2O | - |
Ananas comosus | Benzoyl-Gly + ethanol | - |
? | |
| Benzoyl-L-Arg ethyl ester + H2O | - |
Ananas comosus | Benzoyl-L-Arg + ethanol | - |
? | |
| casein + H2O | - |
Ananas comosus | hydrolyzed casein | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25600, Ananas comosus, enzyme forms SB1 and SB2, SDS-PAGE | Ananas comosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
Benzoyl-Gly ethyl ester | enzyme form SB1 | Ananas comosus | |
| 0.24 | - |
Benzoyl-Gly ethyl ester | enzyme form SB2 | Ananas comosus | |
| 0.39 | - |
benzoyl-L-Arg ethyl ester | enzyme form SB2 | Ananas comosus | |
| 0.42 | - |
benzoyl-L-Arg ethyl ester | enzyme form SB1 | Ananas comosus | |
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