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Literature summary for 3.4.22.32 extracted from
- Characterization of the pineapple stem proteases (bromelains) (1975), Arch. Biochem. Biophys., 167, 678-686.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phenylmercuric acetate | - |
Ananas comosus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18800 | - |
Ananas comosus, bromelain A, gel filtration | Ananas comosus |
| 19700 | - |
Ananas comosus, bromelain B, gel filtration | Ananas comosus |
| 35000 | - |
x * 35000, Ananas comosus, bromelain A, SDS-PAGE | Ananas comosus |
| 35300 | - |
Ananas comosus, sedimentation velocity measurements | Ananas comosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ananas comosus | - |
bromelain A and B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| bromelain A and B | Ananas comosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| stem | - |
Ananas comosus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Ananas comosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzyloxycarbonyl-Gly 4-nitrophenyl ester + H2O | - |
Ananas comosus | Benzyloxycarbonyl-Gly + 4-nitrophenol | - |
? | |
| Benzyloxycarbonyl-L-Ala 4-nitrophenyl ester + H2O | - |
Ananas comosus | Benzyloxycarbonyl-L-Ala + 4-nitrophenol | - |
? | |
| Benzyloxycarbonyl-L-Asn 4-nitrophenyl ester + H2O | - |
Ananas comosus | Benzyloxycarbonyl-L-Asn + 4-nitrophenol | - |
? | |
| Benzyloxycarbonyl-L-Lys 4-nitrophenyl ester + H2O | - |
Ananas comosus | Benzyloxycarbonyl-L-Lys + 4-nitrophenol | - |
? | |
| benzyloxycarbonyl-L-Tyr 4-nitrophenyl ester + H2O | - |
Ananas comosus | benzyloxycarbonyl-L-Tyr + 4-nitrophenol | - |
? | |
| additional information | overview: synthetic substrates | Ananas comosus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, Ananas comosus, bromelain A, SDS-PAGE | Ananas comosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
benzyloxycarbonyl-L-Tyr 4-nitrophenyl ester | bromelain A | Ananas comosus | |
| 0.44 | - |
benzyloxycarbonyl-L-Tyr 4-nitrophenyl ester | bromelain B | Ananas comosus | |
| 1.4 | 1.5 | Benzyloxycarbonyl-L-Asn 4-nitrophenyl ester | - |
Ananas comosus | |
| 1.75 | - |
Benzyloxycarbonyl-Gly 4-nitrophenyl ester | bromelain A | Ananas comosus | |
| 2.5 | - |
benzyloxycarbonyl-L-Ala 4-nitrophenyl ester | bromelain A | Ananas comosus | |
| 3.35 | - |
Benzyloxycarbonyl-Gly 4-nitrophenyl ester | bromelain A | Ananas comosus | |
| 7.4 | - |
benzyloxycarbonyl-L-Lys 4-nitrophenyl ester | bromelain A | Ananas comosus | |
| 8.3 | - |
benzyloxycarbonyl-L-Ala 4-nitrophenyl ester | bromelain B | Ananas comosus | |
| 9.8 | - |
benzyloxycarbonyl-L-Lys 4-nitrophenyl ester | bromelain B | Ananas comosus | |
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Release 2023.1 (January 2023)
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