Application | Comment | Organism |
---|---|---|
analysis | the enzyme should prove generally useful in peptide sequencing | Ficus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester | pH 6.36, 23.2% v/v CH3CN, ficin II | Ficus sp. | |
0.0059 | - |
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester | pH 6.0, 3.17% v/v CH3CN, ficin II | Ficus sp. | |
0.0067 | - |
p-nitrophenyl N-trans-cinnamoylglycinate | ficin III | Ficus sp. | |
0.0076 | - |
p-nitrophenyl N-trans-cinnamoylglycinate | ficin II | Ficus sp. | |
0.0087 | - |
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester | pH 6.0, 3.17% v/v CH3CN, ficin II | Ficus sp. | |
0.012 | - |
N-benzyloxycarbonyl-D-Tyr p-nitrophenyl ester | ficin II | Ficus sp. | |
0.0122 | - |
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester | pH 6.36, 23.2% v/v CH3CN, ficin II | Ficus sp. | |
0.0134 | - |
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester | pH 6.0, 3.17% v/v CH3CN, ficin II | Ficus sp. | |
0.0172 | - |
Nalpha-carbobenzoxy-glycylglycine p-nitrophenyl ester | pH 6.36, 23.2% v/v CH3CN, ficin II | Ficus sp. | |
0.0183 | - |
p-nitrophenyl N-benzyloxycarbonylglycinate | ficin III | Ficus sp. | |
0.0194 | - |
p-nitrophenyl N-benzyloxycarbonylglycinate | ficin II | Ficus sp. | |
0.0214 | - |
p-nitrophenyl N-benzoylglycinate | ficin III | Ficus sp. | |
0.024 | - |
N-benzyloxycarbonyl-D-Leu p-nitrophenyl ester | ficin II | Ficus sp. | |
0.025 | - |
p-nitrophenyl N-benzoylglycinate | ficin II | Ficus sp. | |
0.0258 | - |
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester | pH 6.36, 23.2% v/v CH3CN, ficin II | Ficus sp. | |
0.0327 | - |
Nalpha-carbobenzoxy-Val p-nitrophenyl ester | pH 6.36, 23.2% v/v CH3CN, ficin II | Ficus sp. | |
0.0377 | - |
Nalpha-carbobenzoxy-Phe p-nitrophenyl ester | pH 6.36, 23.2% v/v CH3CN, ficin II | Ficus sp. | |
0.052 | - |
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester | pH 6.36, 23.2% v/v CH3CN, ficin II | Ficus sp. | |
0.0598 | - |
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester | pH 6.36, 23.2% v/v CH3CN, ficin II | Ficus sp. | |
0.128 | - |
N-benzyloxycarbonyl-D-Phe p-nitrophenyl ester | ficin II | Ficus sp. | |
0.2 | - |
N-benzyloxycarbonyl-D-Ala p-nitrophenyl ester | ficin II | Ficus sp. | |
0.309 | - |
p-nitrophenyl N-acetylglycinate | ficin III | Ficus sp. | |
0.328 | - |
p-nitrophenyl N-acetylglycinate | ficin II | Ficus sp. | |
1.1 | - |
p-nitrophenyl N-formylglycinate | ficin III | Ficus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ficus sp. | - |
ficin II and ficin III | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-benzyloxycarbonyl-D-Ala p-nitrophenyl ester + H2O | ficin II | Ficus sp. | N-benzyloxycarbonyl-D-Ala + 4-nitrophenol | - |
? | |
N-benzyloxycarbonyl-D-Leu p-nitrophenyl ester + H2O | ficin II | Ficus sp. | N-benzyloxycarbonyl-D-Leu + 4-nitrophenol | - |
? | |
N-benzyloxycarbonyl-D-Phe p-nitrophenyl ester + H2O | ficin II | Ficus sp. | N-benzyloxycarbonyl-D-Phe + 4-nitrophenol | - |
? | |
N-benzyloxycarbonyl-D-Tyr p-nitrophenyl ester + H2O | ficin II | Ficus sp. | N-benzyloxycarbonyl-D-Tyr + 4-nitrophenol | - |
? | |
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester + H2O | ficin II | Ficus sp. | Nalpha-carbobenzoxy-Ala + 4-nitrophenol | - |
? | |
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester + H2O | ficin II | Ficus sp. | Nalpha-carbobenzoxy-Gly + 4-nitrophenol | - |
? | |
Nalpha-carbobenzoxy-glycylglycine p-nitrophenyl ester + H2O | ficin II | Ficus sp. | ? | - |
? | |
Nalpha-carbobenzoxy-Leu p-nitrophenyl ester + H2O | ficin II | Ficus sp. | Nalpha-carbobenzoxy-Leu + 4-nitrophenol | - |
? | |
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester + H2O | ficin II | Ficus sp. | Nalpha-carbobenzoxy-Lys + 4-nitrophenyl | - |
? | |
Nalpha-carbobenzoxy-Phe p-nitrophenyl ester + H2O | ficin II | Ficus sp. | Nalpha-carbobenzoxy-Phe + 4-nitrophenol | - |
? | |
Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester + H2O | ficin II | Ficus sp. | Nalpha-carbobenzoxy-Tyr + 4-nitrophenol | - |
? | |
Nalpha-carbobenzoxy-Val p-nitrophenyl ester + H2O | ficin II | Ficus sp. | Nalpha-carbobenzoxy-Val + 4-nitrophenyl | - |
? | |
p-nitrophenyl N-acetylglycinate + H2O | ficin II and III | Ficus sp. | p-nitrophenol + N-acetylglycine | - |
? | |
p-nitrophenyl N-benzoylglycinate + H2O | ficin II and III | Ficus sp. | p-nitrophenol + N-benzoylglycine | - |
? | |
p-nitrophenyl N-benzyloxycarbonylglycinate + H2O | ficin II and III | Ficus sp. | p-nitrophenol + N-benzyloxycarbonylglycine | - |
? | |
p-nitrophenyl N-formylglycinate + H2O | ficin II and III | Ficus sp. | p-nitrophenol + N-formylglycine | - |
? | |
p-nitrophenyl N-trans-cinnamoylglycinate + H2O | ficin II and III | Ficus sp. | p-nitrophenol + N-trans-cinnamoylglycine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
N-benzyloxycarbonyl-D-Ala p-nitrophenyl ester | ficin II | Ficus sp. | |
0.92 | - |
N-benzyloxycarbonyl-D-Tyr p-nitrophenyl ester | ficin II | Ficus sp. | |
1.02 | - |
Nalpha-carbobenzoxy-Val p-nitrophenyl ester | pH 6.36, 23.2% v/v CH3CN, ficin II | Ficus sp. | |
1.2 | - |
N-benzyloxycarbonyl-D-Phe p-nitrophenyl ester | ficin II | Ficus sp. | |
1.85 | - |
N-benzyloxycarbonyl-D-Leu p-nitrophenyl ester | ficin II | Ficus sp. | |
2.27 | - |
Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester | pH 6.36, 23.2% v/v CH3CN, ficin II | Ficus sp. | |
2.3 | - |
p-nitrophenyl N-acetylglycinate | ficin II | Ficus sp. | |
2.5 | - |
p-nitrophenyl N-formylglycinate | ficin II | Ficus sp. | |
2.83 | - |
Nalpha-carbobenzoxy-Phe p-nitrophenyl ester | pH 6.36, 23.2% v/v CH3CN, ficin II | Ficus sp. | |
2.84 | - |
p-nitrophenyl N-acetylglycinate | ficin III | Ficus sp. | |
4.5 | - |
Nalpha-carbobenzoxy-Leu p-nitrophenyl ester | pH 6.36, 23.2% v/v CH3CN, ficin II | Ficus sp. | |
7.45 | - |
Nalpha-carbobenzoxy-glycylglycine p-nitrophenyl ester | pH 6.36, 23.2% v/v CH3CN, ficin II | Ficus sp. | |
7.72 | - |
p-nitrophenyl N-benzoylglycinate | ficin III | Ficus sp. | |
9.25 | - |
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester | pH 6.0, 3.17% v/v CH3CN, ficin II | Ficus sp. | |
12.4 | - |
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester | pH 6.0, 3.17% v/v CH3CN, ficin II | Ficus sp. | |
13.3 | - |
p-nitrophenyl N-benzyloxycarbonylglycinate | ficin III | Ficus sp. | |
29.8 | - |
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester | pH 6.36, 23.2% v/v CH3CN, ficin II | Ficus sp. | |
31 | - |
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester | pH 6.36, 23.2% v/v CH3CN, ficin II | Ficus sp. | |
35 | - |
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester | pH 6.0, 3.17% v/v CH3CN, ficin II | Ficus sp. | |
53.6 | - |
p-nitrophenyl N-benzoylglycinate | ficin II | Ficus sp. | |
55 | - |
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester | pH 6.36, 23.2% v/v CH3CN, ficin II | Ficus sp. | |
86.4 | - |
p-nitrophenyl N-trans-cinnamoylglycinate | ficin II | Ficus sp. | |
95.7 | - |
p-nitrophenyl N-benzyloxycarbonylglycinate | ficin III | Ficus sp. | |
123 | - |
p-nitrophenyl N-trans-cinnamoylglycinate | ficin III | Ficus sp. |