Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | the enzyme requires activation by reducing agents, optimal at 4 mM | Ficus glabrata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cystatin | from egg white, strong inhibition | Ficus glabrata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
benzoyl-Phe-Val-Arg-NH-7-amido-4-methylcoumarin | pH 6.8, 37°C | Ficus glabrata | |
0.3 | - |
benzyloxycarbonyl-Phe-Arg-NH-(4-nitrophenyl) | pH 6.8, 37°C | Ficus glabrata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Ficus glabrata | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ficus glabrata | ficain is the major proteolytic activity component in the milky latex of the plant | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ficus glabrata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
further purification of a commercial latex preparation by cation exchange chromatography, and 2,2'-dipyridyl disulfide-glutathione or aminophenylmercuric acetate affinity chromatography, or by Gly-Phe-NH-methylcyanide affinity chromatography with elution by 2,2'-dipyridyl disulfide | Ficus glabrata |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Similar to that of papain | active site structure | Ficus glabrata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | needs further purification | Ficus glabrata | - |
fruit | unripe, the enzyme is located in the latex milk secreted by the organ | Ficus glabrata | - |
latex | - |
Ficus glabrata | - |
leaf | the enzyme is located in the latex milk secreted by the organ | Ficus glabrata | - |
stem | the enzyme is located in the latex milk secreted by the organ | Ficus glabrata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-Phe-Val-Arg-NH-(4-nitrophenyl) + H2O | - |
Ficus glabrata | benzoyl-Phe-Val-Arg + 4-nitrophenol | - |
? | |
benzoyl-Phe-Val-Arg-NH-7-amido-4-methylcoumarin + H2O | - |
Ficus glabrata | benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Phe-Arg-NH-(4-nitrophenyl) + H2O | - |
Ficus glabrata | benzyloxycarbonyl-Phe-Arg + 4-nitrophenol | - |
? | |
benzyloxycarbonyl-Phe-Arg-NH-7-amido-4-methylcoumarin + H2O | - |
Ficus glabrata | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
insulin B chain + H2O | hydrolysis of 6 bonds | Ficus glabrata | fragments of insulin B chain | - |
? | |
additional information | ficain is the major proteolytic activity component in the milky latex of the plant | Ficus glabrata | ? | - |
? | |
additional information | substrate specificity compared to papain, the ficain S1-pocket accepts Gly, SEr, Glu, Tyr, and Phe, the S2-pocket accepts cysteic acid, Val, Leu, Gly, and Phe, Arg-NH-nitrophenyl is a poor substrate | Ficus glabrata | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | amino acid sequence sand structure analysis | Ficus glabrata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Ficus glabrata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile | Ficus glabrata |