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Literature summary for 3.4.22.3 extracted from

  • Ruemenapf, T.; Thiel, H.J.
    434. Cathepsin T (2004), Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press, 2nd Ed., 1404.
No PubMed abstract available
Search for more literature for 3.4.22.3

Activating Compound

Activating Compound Comment Organism Structure
cysteine the enzyme requires activation by reducing agents, optimal at 4 mM Ficus glabrata

Inhibitors

Inhibitors Comment Organism Structure
cystatin from egg white, strong inhibition Ficus glabrata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
 benzoyl-Phe-Val-Arg-NH-7-amido-4-methylcoumarin pH 6.8, 37°C Ficus glabrata
0.3
-
 benzyloxycarbonyl-Phe-Arg-NH-(4-nitrophenyl) pH 6.8, 37°C Ficus glabrata

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Ficus glabrata
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Ficus glabrata ficain is the major proteolytic activity component in the milky latex of the plant ?
-
 ?

Organism

Organism UniProt Comment Textmining
Ficus glabrata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
further purification of a commercial latex preparation by cation exchange chromatography, and 2,2'-dipyridyl disulfide-glutathione or aminophenylmercuric acetate affinity chromatography, or by Gly-Phe-NH-methylcyanide affinity chromatography with elution by 2,2'-dipyridyl disulfide Ficus glabrata

Reaction

Reaction Comment Organism Reaction ID
Similar to that of papain active site structure Ficus glabrata
a

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation needs further purification Ficus glabrata
-
fruit unripe, the enzyme is located in the latex milk secreted by the organ Ficus glabrata
-
latex
-
 Ficus glabrata
-
leaf the enzyme is located in the latex milk secreted by the organ Ficus glabrata
-
stem the enzyme is located in the latex milk secreted by the organ Ficus glabrata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Phe-Val-Arg-NH-(4-nitrophenyl) + H2O
-
 Ficus glabrata benzoyl-Phe-Val-Arg + 4-nitrophenol
-
 ?
benzoyl-Phe-Val-Arg-NH-7-amido-4-methylcoumarin + H2O
-
 Ficus glabrata benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Arg-NH-(4-nitrophenyl) + H2O
-
 Ficus glabrata benzyloxycarbonyl-Phe-Arg + 4-nitrophenol
-
 ?
benzyloxycarbonyl-Phe-Arg-NH-7-amido-4-methylcoumarin + H2O
-
 Ficus glabrata benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
insulin B chain + H2O hydrolysis of 6 bonds Ficus glabrata fragments of insulin B chain
-
 ?
additional information ficain is the major proteolytic activity component in the milky latex of the plant Ficus glabrata ?
-
 ?
additional information substrate specificity compared to papain, the ficain S1-pocket accepts Gly, SEr, Glu, Tyr, and Phe, the S2-pocket accepts cysteic acid, Val, Leu, Gly, and Phe, Arg-NH-nitrophenyl is a poor substrate Ficus glabrata ?
-
 ?

Subunits

Subunits Comment Organism
More amino acid sequence sand structure analysis Ficus glabrata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Ficus glabrata

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 pH profile Ficus glabrata