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Literature summary for 3.4.22.28 extracted from

  • Youell, J.; Fordham, D.; Firman, K.
    Production and single-step purification of EGFP and a biotinylated version of the Human Rhinovirus 14 3C protease (2011), Protein Expr. Purif., 79, 258-262.
    View publication on PubMed

Application

Application Comment Organism
molecular biology a protein-tagging system for purification of EGFP from Escherichia coli using a single-step glutathione column purification and release of EGFP from the column using a variant of Human Rhinovirus 14 3C is described. A biotinylated variant of Human Rhinovirus 14 3C protease (bHR3Cp) is constructed, which includes a factor Xa or thrombin cleavage site between the protease gene and the biotinylation signal. This system readily allows attachment of the protease to a variety of surfaces and subsequent release using either thrombin or factor Xa proteases Rhinovirus B14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rhinovirus B14

Organism

Organism UniProt Comment Textmining
Rhinovirus B14
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a DEAE column Rhinovirus B14

Synonyms

Synonyms Comment Organism
3C protease
-
Rhinovirus B14
3Cpro
-
Rhinovirus B14