Application | Comment | Organism |
---|---|---|
molecular biology | a protein-tagging system for purification of EGFP from Escherichia coli using a single-step glutathione column purification and release of EGFP from the column using a variant of Human Rhinovirus 14 3C is described. A biotinylated variant of Human Rhinovirus 14 3C protease (bHR3Cp) is constructed, which includes a factor Xa or thrombin cleavage site between the protease gene and the biotinylation signal. This system readily allows attachment of the protease to a variety of surfaces and subsequent release using either thrombin or factor Xa proteases | Rhinovirus B14 |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Rhinovirus B14 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhinovirus B14 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a DEAE column | Rhinovirus B14 |
Synonyms | Comment | Organism |
---|---|---|
3C protease | - |
Rhinovirus B14 |
3Cpro | - |
Rhinovirus B14 |