Cloned (Comment) | Organism |
---|---|
cloned and expressed in Escherichia coli as a His-tagged fusion protein | Enterovirus B93 |
Crystallization (Comment) | Organism |
---|---|
crystal structure of 3Cpro alone and in complex with the anti-HRV molecules compound (AG7404) and rupintrivir (AG7088) at resolutions of 1.9, 1.3, and 1.5 A is reported. This 20.2-kDa cysteine protease adopts a chymotrypsin-like fold with a catalytic triad, Cys147-His40-Glu71, located in the cleft between the two six-stranded barrels. The residues that form the inhibitor binding pockets are highly conserved among EV 3Cpros, which explains the broad activity of compounds developed initially against a specific virus | Enterovirus B93 |
Protein Variants | Comment | Organism |
---|---|---|
C147A | inactive mutant | Enterovirus B93 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethyl (2E,4S)-4-([(2S)-2-[3-[[(5-methyl-1,2-oxazol-3-yl)carbonyl]amino]-2-oxopyridin-1(2H)-yl]pent-4-ynoyl]amino)-5-[(3R)-2-oxopyrrolidin-3-yl]pent-2-enoate | compound AG7404 | Enterovirus B93 | |
rupintrivir | - |
Enterovirus B93 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterovirus B93 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Enterovirus B93 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-RHSVGATLEALFQGPPVYREIKIS-NH2 + H2O | - |
Enterovirus B93 | Ac-RHSVGATLEALFQ + GPPVYREIKIS-NH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3C protease | - |
Enterovirus B93 |
3Cpro | - |
Enterovirus B93 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Enterovirus B93 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Enterovirus B93 |