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Literature summary for 3.4.22.28 extracted from

  • Hussey, R.J.; Coates, L.; Gill, R.S.; Erskine, P.T.; Coker, S.F.; Mitchell, E.; Cooper, J.B.; Wood, S.; Broadbridge, R.; Clarke, I.N.; Lambden, P.R.; Shoolingin-Jordan, P.M.
    A Structural Study of Norovirus 3C Protease Specificity: Binding of a Designed Active Site-Directed Peptide Inhibitor (2010), Biochemistry, 50, 240-249.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.28

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray structure of the Southampton norovirus 3C protease (SV3CP) bound to an active site-directed peptide inhibitor which has been refined at 1.7 A resolution is described Southampton virus

Inhibitors

Inhibitors Comment Organism Structure
N-acetyl-L-alpha-glutamyl-L-phenylalanyl-L-glutaminyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-L-leucinamide i.e. Michael acceptor peptidyl inhibitor (MAPI). Irreversible inhibitor in which the natural polyprotein substrate recognition sequence is linked to a propenyl ethyl ester moiety (X). The catalytic cysteine at position 139 of the enzyme is rapidly modified by the inhibitor Southampton virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 Ac-EFQLQ-4-nitroanilide pH 8.5, temperature not specified in the publication Southampton virus
0.8
-
 Ac-DEFQLQ-4-nitroanilide pH 8.5, temperature not specified in the publication Southampton virus
1.5
-
 Ac-FQLQ-4-nitroanilide pH 8.5, temperature not specified in the publication Southampton virus

Organism

Organism UniProt Comment Textmining
Southampton virus Q04544
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-DEFQLQ-4-nitroanilide + H2O
-
 Southampton virus ?
-
 ?
Ac-EFQLQ-4-nitroanilide + H2O
-
 Southampton virus ?
-
 ?
Ac-FQLQ-4-nitroanilide + H2O
-
 Southampton virus ?
-
 ?

Synonyms

Synonyms Comment Organism
3C protease
-
 Southampton virus
SV3CP
-
 Southampton virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 Ac-FQLQ-4-nitroanilide pH 8.5, temperature not specified in the publication Southampton virus
0.14
-
 Ac-EFQLQ-4-nitroanilide pH 8.5, temperature not specified in the publication Southampton virus
0.33
-
 Ac-DEFQLQ-4-nitroanilide pH 8.5, temperature not specified in the publication Southampton virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Southampton virus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.05
-
 Ac-FQLQ-4-nitroanilide pH 8.5, temperature not specified in the publication Southampton virus
0.41
-
 Ac-DEFQLQ-4-nitroanilide pH 8.5, temperature not specified in the publication Southampton virus
0.46
-
 Ac-EFQLQ-4-nitroanilide pH 8.5, temperature not specified in the publication Southampton virus