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Literature summary for 3.4.22.28 extracted from

  • Zunszain, P.A.; Knox, S.R.; Sweeney, T.R.; Yang, J.; Roque-Rosell, N.; Belsham, G.J.; Leatherbarrow, R.J.; Curry, S.
    Insights into cleavage specificity from the crystal structure of foot-and-mouth disease virus 3C protease complexed with a peptide substrate (2010), J. Mol. Biol., 395, 375-389.
    View publication on PubMed
Search for more literature for 3.4.22.28

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Foot-and-mouth disease virus

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with substrate APAKQLLNFD, sitting drop vapor diffusion method, using 40-42.5% (w/v)polyethylene glycol 400, 0.2 M LiSO4 and 0.1 M Tris (pH 8.0), and in complex with substrate APAKELLNFD, sitting drop vapor diffusion method, using 41-43% (w/v) polyethylene glycol 400, 0.2 M LiSO4 and 0.1 M Tris (pH 8.0) Foot-and-mouth disease virus

Protein Variants

Protein Variants Comment Organism
C142L introduction of the C142L mutation into a type O1K FMDV has no significant effect on the infectivity of the virus in tissue culture Foot-and-mouth disease virus
C163A inactive Foot-and-mouth disease virus
C31A the single mutation reduces the rate of cleavage of the APAKQLLNFD peptide by about 3fold Foot-and-mouth disease virus
C31A/L47A the double mutation causes only a very slight further reduction in cleavage activity of the APAKQLLNFD peptide compared to the single mutation C31A Foot-and-mouth disease virus
D84E cleavage by the D84E mutant is about 200times slower than cleavage by wild type enzyme Foot-and-mouth disease virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
picornavirus polyprotein + H2O Foot-and-mouth disease virus
-
 hydrolyzed picornavirus polyprotein
-
 ?

Organism

Organism UniProt Comment Textmining
Foot-and-mouth disease virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon beads chromatography Foot-and-mouth disease virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
APAKELLNFD + H2O
-
 Foot-and-mouth disease virus APAKE + LLNFD
-
 ?
APAKQLLNFD + H2O
-
 Foot-and-mouth disease virus APAKQ + LLNFD
-
 ?
additional information the foot-and-mouth disease virus 3Cpro to cleaves sequences containing either P1-Gln or P1-Glu Foot-and-mouth disease virus ?
-
 ?
picornavirus polyprotein + H2O
-
 Foot-and-mouth disease virus hydrolyzed picornavirus polyprotein
-
 ?

Synonyms

Synonyms Comment Organism
3C protease
-
 Foot-and-mouth disease virus
3CP
-
 Foot-and-mouth disease virus