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Literature summary for 3.4.22.28 extracted from

  • Bonderoff, J.M.; Larey, J.L.; Lloyd, R.E.
    Cleavage of poly(A)-binding protein by poliovirus 3C proteinase inhibits viral internal ribosome entry site-mediated translation (2008), J. Virol., 82, 9389-9399.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.28

Cloned(Commentary)

Cloned (Comment) Organism
poliovirus His-3Cpro is expressed in Escherichia coli from pET-3C Enterovirus C

Protein Variants

Protein Variants Comment Organism
C147A catalytically inactive 3C proteinase has only a modest effect on translation when high doses are added to lysates, and proteinase that is heat inactivated at 70°C for 15 min has no significant effect on translation Enterovirus C

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly(A)-binding protein + H2O Enterovirus C inhibition of viral internal ribosome entry site-mediated translation, cleavage of poly(A)-binding protein by 3Cpro is a necessary component for host translation shutoff fragments of poly(A)-binding protein
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 ?

Organism

Organism UniProt Comment Textmining
Enterovirus C
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-3Cpro is purified using Ni-nitrilotriacetic acid chelating resin under non-denaturing conditions Enterovirus C

Source Tissue

Source Tissue Comment Organism Textmining
infected cell
-
 Enterovirus C
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 3Cpro inhibits cap-independent translation mediated by the poliovirus internal ribosome entry site in a dose-dependent manner in HeLa translation extracts displaying cap-poly(A) synergy, 3Cpro-induced translation inhibition can be partially rescued by addition of recombinant poly(A)-binding protein in vitro Enterovirus C
additional information
-
 combined 2Apro and 3Cpro result in a net stimulation of poliovirus internal ribosome entry site-mediated translation, the resulting rate of translation is about 3fold greater than the control value but not as great as the 4fold stimulation of translation from preincubation with 2Apro alone Enterovirus C

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A)-binding protein + H2O inhibition of viral internal ribosome entry site-mediated translation, cleavage of poly(A)-binding protein by 3Cpro is a necessary component for host translation shutoff Enterovirus C fragments of poly(A)-binding protein
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 ?

Synonyms

Synonyms Comment Organism
3C proteinase
-
 Enterovirus C
3Cpro
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 Enterovirus C

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Enterovirus C

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Enterovirus C