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Literature summary for 3.4.22.28 extracted from

  • Banerjee, R.; Weidman, M.K.; Echeverri, A.; Kundu, P.; Dasgupta, A.
    Regulation of poliovirus 3C protease by the 2C polypeptide (2004), J. Virol., 78, 9243-9256.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine poliovirus-encoded nonstructural polypeptide 2C can negatively regulate the viral protease 3Cpro-mediated cleavage of transcription factor cyclic AMP-responsive element binding protein in vitro Enterovirus C

Cloned(Commentary)

Cloned (Comment) Organism
into pQE30 and expressed in Escherichia coli M15 pREP4 strain Enterovirus C

Protein Variants

Protein Variants Comment Organism
additional information 2C deletion mutants affect its ability to inhibit 3Cpro Enterovirus C

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by the viral RNA polymerase 3Dpol Enterovirus C
poliovirus-encoded nonstructural polypeptide 2B low but detectable activity Enterovirus C
poliovirus-encoded nonstructural polypeptide 2BC efficiently blocks 3Cpro activity Enterovirus C
poliovirus-encoded nonstructural polypeptide 2C efficiently blocks 3Cpro activity, inhibits 3Cpro-catalyzed cleavage of cellular transcription factors at Q-G sites in vitro Enterovirus C

Organism

Organism UniProt Comment Textmining
Enterovirus C
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to near homogeneity Enterovirus C

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
in vitro-translated (35S)methionine-labeled transcription factor cyclic AMP-responsive element binding protein + H2O
-
Enterovirus C ?
-
?
RNA polymerase I factor SL-1 + H2O
-
Enterovirus C ?
-
?
TATA-binding protein + H2O
-
Enterovirus C ?
-
?
viral 3CD protein + H2O
-
Enterovirus C ?
-
?

Synonyms

Synonyms Comment Organism
3C protease
-
Enterovirus C
3Cpro
-
Enterovirus C