Application | Comment | Organism |
---|---|---|
medicine | poliovirus-encoded nonstructural polypeptide 2C can negatively regulate the viral protease 3Cpro-mediated cleavage of transcription factor cyclic AMP-responsive element binding protein in vitro | Enterovirus C |
Cloned (Comment) | Organism |
---|---|
into pQE30 and expressed in Escherichia coli M15 pREP4 strain | Enterovirus C |
Protein Variants | Comment | Organism |
---|---|---|
additional information | 2C deletion mutants affect its ability to inhibit 3Cpro | Enterovirus C |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by the viral RNA polymerase 3Dpol | Enterovirus C | |
poliovirus-encoded nonstructural polypeptide 2B | low but detectable activity | Enterovirus C | |
poliovirus-encoded nonstructural polypeptide 2BC | efficiently blocks 3Cpro activity | Enterovirus C | |
poliovirus-encoded nonstructural polypeptide 2C | efficiently blocks 3Cpro activity, inhibits 3Cpro-catalyzed cleavage of cellular transcription factors at Q-G sites in vitro | Enterovirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterovirus C | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to near homogeneity | Enterovirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
in vitro-translated (35S)methionine-labeled transcription factor cyclic AMP-responsive element binding protein + H2O | - |
Enterovirus C | ? | - |
? | |
RNA polymerase I factor SL-1 + H2O | - |
Enterovirus C | ? | - |
? | |
TATA-binding protein + H2O | - |
Enterovirus C | ? | - |
? | |
viral 3CD protein + H2O | - |
Enterovirus C | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3C protease | - |
Enterovirus C |
3Cpro | - |
Enterovirus C |