Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Hepatovirus A |
Crystallization (Comment) | Organism |
---|---|
double mutant C24S/C172A | Hepatovirus A |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
1 * 24000, SDS-PAGE, under reducing conditions, wild-type and C172A mutant enzyme each behave as dimers under nonreducing conditions | Hepatovirus A |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepatovirus A | - |
recombinant enzyme | - |
Hepatovirus A | - |
C172A and C24S/C172A | - |
Purification (Comment) | Organism |
---|---|
recombinant enzymes | Hepatovirus A |
wild-type and 3 mutant enzymes | Hepatovirus A |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 24000, SDS-PAGE, under reducing conditions, wild-type and C172A mutant enzyme each behave as dimers under nonreducing conditions | Hepatovirus A |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Hepatovirus A | isoform D from wild-type and active mutant C24S | - |
8.35 |
Hepatovirus A | isoform C from wild-type and active mutant C24S and isoforms C from mutants C24S/C172A and C172A | - |
8.65 |
Hepatovirus A | isoform B from wild-type and active mutant C24S | - |
8.85 |
Hepatovirus A | isoform A from wild-type and active mutant C24S and isoforms A from mutants C24S/C172A and C172A | - |
9.1 |